Ahmadi-Motamayel Fatemeh, Goodarzi Mohammad T, Jamshidi Zohreh, Kebriaei Reza
Department of Oral Medicine, Dental Research Center and Research Center for Molecular Medicine, Hamadan University of Medical SciencesHamadan, Iran.
Research Center for Molecular Medicine, Hamadan University of Medical SciencesHamadan, Iran.
Front Physiol. 2017 Mar 31;8:189. doi: 10.3389/fphys.2017.00189. eCollection 2017.
Local bacteria stimulate polymorphonuclear neutrophils to release reactive oxygen species in periodontitis. Increased levels of oxidative stress play a significant role in the pathogenesis of periodontitis. Therefore, this study aimed to evaluate total salivary and serum antioxidant capacity and malondialdehyde in patients with chronic periodontitis. Fifty-five healthy subjects and 55 patients with chronic periodontitis, with an age range of 30-50 years, were evaluated. After clinical examination and case selection, unstimulated whole saliva was collected in the morning. Blood samples were taken from the antecubital vein. Total antioxidant capacity and malondialdehyde levels were evaluated by spectrophotometric assay. Data were analyzed with -test, using Stata.11 software program. The periodontitis group exhibited lower salivary (0.16) and serum (0.36) total antioxidant capacity ( = 0.11) compared to the control group. Mean salivary malondialdehyde levels in the case and control groups were 0.80 ± 0.09 and 0.42 ± 0.08, respectively. The results showed significantly higher levels of salivary and serum malondialdehyde in the periodontitis group. Gender did not have any effect on antioxidant and oxidative stress levels. This study indicated increased levels of salivary and serum oxidative stresses in patients with chronic periodontitis. Total antioxidant capacity was mildly lower in the saliva and serum of these patients. Higher malondialdehyde levels with no changes in antioxidant status can result in systemic and local complications in these patients.
局部细菌刺激多形核中性粒细胞在牙周炎中释放活性氧。氧化应激水平升高在牙周炎发病机制中起重要作用。因此，本研究旨在评估慢性牙周炎患者唾液和血清的总抗氧化能力及丙二醛水平。对55名年龄在30至50岁之间的健康受试者和55名慢性牙周炎患者进行了评估。经过临床检查和病例筛选后，于早晨采集未刺激的全唾液。从前臂静脉采集血样。通过分光光度法测定总抗氧化能力和丙二醛水平。使用Stata.11软件程序进行t检验分析数据。与对照组相比，牙周炎组唾液（0.16）和血清（0.36）的总抗氧化能力较低（P = 0.11）。病例组和对照组唾液丙二醛的平均水平分别为0.80±0.09和0.42±0.08。结果显示，牙周炎组唾液和血清中的丙二醛水平显著更高。性别对抗氧化和氧化应激水平没有任何影响。本研究表明，慢性牙周炎患者唾液和血清中的氧化应激水平升高。这些患者唾液和血清中的总抗氧化能力略有降低。丙二醛水平升高而抗氧化状态无变化可能导致这些患者出现全身和局部并发症。
