• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

成熟脂肪细胞去分化过程中基因表达谱的时间变化

Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation.

作者信息

Côté Julie Anne, Guénard Frédéric, Lessard Julie, Lapointe Marc, Biron Simon, Vohl Marie-Claude, Tchernof André

机构信息

Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, Canada G1V 4G5.

Centre de Recherche du CHU de Québec, 2705 Boulevard Laurier, Quebec City, QC, Canada G1V 4G2.

出版信息

Int J Genomics. 2017;2017:5149362. doi: 10.1155/2017/5149362. Epub 2017 Mar 19.

DOI:10.1155/2017/5149362
PMID:28409151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5376413/
Abstract

. To characterize changes in gene expression profile during human mature adipocyte dedifferentiation in ceiling culture. . Subcutaneous (SC) and omental (OM) adipose tissue samples were obtained from 4 participants paired for age and BMI. Isolated adipocytes were dedifferentiated in ceiling culture. Gene expression analysis at days 0, 4, 7, and 12 of the cultures was performed using Affymetrix Human Gene 2.0 STvi arrays. Hierarchical clustering according to similarity of expression changes was used to identify overrepresented functions. . Four clusters gathered genes with similar expression between day 4 to day 7 but decreasing expression from day 7 to day 12. Most of these genes coded for proteins involved in adipocyte functions (, , , , , , , , , , and ). Expression of several genes coding for proteins implicated in cellular proliferation and growth or cell cycle increased significantly from day 7 to day 12 (, , and ). Genes coding for extracellular matrix proteins were differentially expressed between days 0, 4, 7, and 12 (, , and , , and ). . Dedifferentiation is associated with downregulation of transcripts encoding proteins involved in mature adipocyte functions and upregulation of genes involved in matrix remodeling, cellular development, and cell cycle.

摘要

为了表征在悬滴培养中人类成熟脂肪细胞去分化过程中的基因表达谱变化。从4名年龄和体重指数相匹配的参与者身上获取皮下（SC）和网膜（OM）脂肪组织样本。分离出的脂肪细胞在悬滴培养中进行去分化。使用Affymetrix Human Gene 2.0 STvi阵列对培养第0、4、7和12天的基因表达进行分析。根据表达变化的相似性进行层次聚类，以识别过度表达的功能。四个聚类聚集了在第4天到第7天之间表达相似但从第7天到第12天表达下降的基因大多这些基因编码参与脂肪细胞功能的蛋白质（……）。从第7天到第12天，几个编码参与细胞增殖、生长或细胞周期的蛋白质的基因表达显著增加（……）。编码细胞外基质蛋白的基因在第0、4、7和12天之间存在差异表达（……）。去分化与编码参与成熟脂肪细胞功能的蛋白质的转录本下调以及参与基质重塑、细胞发育和细胞周期的基因上调有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/f42693d617ad/IJG2017-5149362.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/2c679a2b6c5c/IJG2017-5149362.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/14267dbf5edf/IJG2017-5149362.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/a6425bb28250/IJG2017-5149362.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/dd89e1895900/IJG2017-5149362.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/78ff93502943/IJG2017-5149362.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/e7dc0a971c3a/IJG2017-5149362.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/f42693d617ad/IJG2017-5149362.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/2c679a2b6c5c/IJG2017-5149362.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/14267dbf5edf/IJG2017-5149362.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/a6425bb28250/IJG2017-5149362.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/dd89e1895900/IJG2017-5149362.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/78ff93502943/IJG2017-5149362.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/e7dc0a971c3a/IJG2017-5149362.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6209/5376413/f42693d617ad/IJG2017-5149362.007.jpg

相似文献

1
Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation.成熟脂肪细胞去分化过程中基因表达谱的时间变化
Int J Genomics. 2017;2017:5149362. doi: 10.1155/2017/5149362. Epub 2017 Mar 19.
2
Role of the TGF-β pathway in dedifferentiation of human mature adipocytes.转化生长因子-β信号通路在人成熟脂肪细胞去分化中的作用
FEBS Open Bio. 2017 Jul 10;7(8):1092-1101. doi: 10.1002/2211-5463.12250. eCollection 2017 Aug.
3
Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.来自内脏和皮下脂肪组织的去分化人成熟脂肪细胞的特征：成纤维细胞激活蛋白α和二肽基肽酶4作为基质重塑的主要成分
PLoS One. 2015 Mar 27;10(3):e0122065. doi: 10.1371/journal.pone.0122065. eCollection 2015.
4
Depot-related gene expression in human subcutaneous and omental adipocytes.人体皮下和网膜脂肪细胞中与储存库相关的基因表达。
Diabetes. 1998 Sep;47(9):1384-91. doi: 10.2337/diabetes.47.9.1384.
5
Characterization and visualization of the liposecretion process taking place during ceiling culture of human mature adipocytes.人成熟脂肪细胞天花板培养过程中脂滴分泌的特征化和可视化。
J Cell Physiol. 2019 Jul;234(7):10270-10280. doi: 10.1002/jcp.27931. Epub 2018 Dec 18.
6
Abdominal subcutaneous and omental adipocyte morphology and its relation to gene expression, lipolysis and adipocytokine levels in women.女性腹部皮下和网膜脂肪细胞形态及其与基因表达、脂肪分解和脂肪细胞因子水平的关系。
Metabolism. 2014 Mar;63(3):372-81. doi: 10.1016/j.metabol.2013.11.007. Epub 2013 Nov 21.
7
Using gene expression to predict differences in the secretome of human omental vs. subcutaneous adipose tissue.利用基因表达预测人网膜与皮下脂肪组织分泌组的差异。
Obesity (Silver Spring). 2012 Jun;20(6):1158-67. doi: 10.1038/oby.2012.14. Epub 2012 Jan 28.
8
[Experimental study of human adipocyte dedifferentiation for adipose tissue engineering].用于脂肪组织工程的人脂肪细胞去分化的实验研究
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Apr;29(4):606-10.
9
Influence of adipocyte size and adipose depot on the number of adipose tissue macrophages and the expression of adipokines in dairy cows at the end of pregnancy.妊娠末期奶牛脂肪细胞大小和脂肪组织对脂肪组织巨噬细胞数量和 adipokines 表达的影响。
J Dairy Sci. 2018 Jul;101(7):6542-6555. doi: 10.3168/jds.2017-13777. Epub 2018 Apr 5.
10
Expression of adipokine and lipid metabolism genes in adipose tissue of dairy cows differing in a female fertility quantitative trait locus.奶牛脂肪组织中与雌性生育力数量性状位点相关的脂肪因子和脂质代谢基因的表达。
J Dairy Sci. 2013;96(12):7591-602. doi: 10.3168/jds.2013-6615. Epub 2013 Oct 11.

引用本文的文献

1
Exploring the role of cell cycle regulation in human mature adipocyte dedifferentiation.探索细胞周期调控在人类成熟脂肪细胞去分化中的作用。
Front Cell Dev Biol. 2025 May 8;13:1547836. doi: 10.3389/fcell.2025.1547836. eCollection 2025.
2
The Impact of Resident Adipose Tissue Macrophages on Adipocyte Homeostasis and Dedifferentiation.驻留脂肪组织巨噬细胞对脂肪细胞稳态和去分化的影响。
Int J Mol Sci. 2024 Dec 4;25(23):13019. doi: 10.3390/ijms252313019.
3
Insights into the molecular changes of adipocyte dedifferentiation and its future research opportunities.

本文引用的文献

1
Human White Adipocytes Convert Into "Rainbow" Adipocytes In Vitro.人白色脂肪细胞在体外转化为“彩虹”脂肪细胞。
J Cell Physiol. 2017 Oct;232(10):2887-2899. doi: 10.1002/jcp.25743. Epub 2017 Apr 12.
2
Adiponectin, the past two decades.脂联素，过去二十年。 （这段原文似乎不太完整，翻译出来的内容可能不太符合完整语境的准确意思）
J Mol Cell Biol. 2016 Apr;8(2):93-100. doi: 10.1093/jmcb/mjw011. Epub 2016 Mar 18.
3
Adipocytes WNT5a mediated dedifferentiation: a possible target in pancreatic cancer microenvironment.脂肪细胞WNT5a介导的去分化：胰腺癌微环境中的一个可能靶点。
浅析脂肪细胞去分化的分子变化及其未来的研究机遇。
J Lipid Res. 2024 Nov;65(11):100644. doi: 10.1016/j.jlr.2024.100644. Epub 2024 Sep 18.
4
Screening for genes, miRNAs and transcription factors of adipogenic differentiation and dedifferentiation of mesenchymal stem cells.筛选与间充质干细胞成脂分化和去分化相关的基因、miRNA 和转录因子。
J Orthop Surg Res. 2023 Jan 17;18(1):46. doi: 10.1186/s13018-023-03514-0.
5
Deep Small RNA Sequencing Reveals Important miRNAs Related to Muscle Development and Intramuscular Fat Deposition in Muscle From Different Goat Breeds.深度小RNA测序揭示了与不同山羊品种肌肉发育和肌内脂肪沉积相关的重要微小RNA
Front Vet Sci. 2022 Jun 13;9:911166. doi: 10.3389/fvets.2022.911166. eCollection 2022.
6
Overview of the molecular mechanisms contributing to the formation of cancer‑associated adipocytes (Review).癌症相关脂肪细胞形成的分子机制概述（综述）。
Mol Med Rep. 2021 Nov;24(5). doi: 10.3892/mmr.2021.12408. Epub 2021 Sep 7.
7
COL6A3 expression in adipose tissue cells is associated with levels of the homeobox transcription factor PRRX1.COL6A3 在脂肪组织细胞中的表达与同源盒转录因子 PRRX1 的水平相关。
Sci Rep. 2020 Nov 19;10(1):20164. doi: 10.1038/s41598-020-77406-2.
8
Engineering 3D degradable, pliable scaffolds toward adipose tissue regeneration; optimized printability, simulations and surface modification.工程化3D可降解、柔韧支架用于脂肪组织再生；优化的可打印性、模拟及表面改性
J Tissue Eng. 2020 Sep 16;11:2041731420954316. doi: 10.1177/2041731420954316. eCollection 2020 Jan-Dec.
9
Insulin negatively regulates dedifferentiation of mouse adipocytes in vitro.胰岛素负调控体外培养的小鼠脂肪细胞去分化。
Adipocyte. 2020 Dec;9(1):24-34. doi: 10.1080/21623945.2020.1721235.
10
Role of the TGF-β pathway in dedifferentiation of human mature adipocytes.转化生长因子-β信号通路在人成熟脂肪细胞去分化中的作用
FEBS Open Bio. 2017 Jul 10;7(8):1092-1101. doi: 10.1002/2211-5463.12250. eCollection 2017 Aug.
Oncotarget. 2016 Apr 12;7(15):20223-35. doi: 10.18632/oncotarget.7936.
4
Evidence of β-Cell Dedifferentiation in Human Type 2 Diabetes.人类2型糖尿病中β细胞去分化的证据。
J Clin Endocrinol Metab. 2016 Mar;101(3):1044-54. doi: 10.1210/jc.2015-2860. Epub 2015 Dec 29.
5
Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.来自内脏和皮下脂肪组织的去分化人成熟脂肪细胞的特征：成纤维细胞激活蛋白α和二肽基肽酶4作为基质重塑的主要成分
PLoS One. 2015 Mar 27;10(3):e0122065. doi: 10.1371/journal.pone.0122065. eCollection 2015.
6
Prostaglandin (PG) F2 alpha synthesis in human subcutaneous and omental adipose tissue: modulation by inflammatory cytokines and role of the human aldose reductase AKR1B1.人皮下和网膜脂肪组织中前列腺素（PG）F2α的合成：炎性细胞因子的调节作用及人醛糖还原酶AKR1B1的作用
PLoS One. 2014 Mar 24;9(3):e90861. doi: 10.1371/journal.pone.0090861. eCollection 2014.
7
Pluripotent stem cells derived from mouse and human white mature adipocytes.源自小鼠和人白色成熟脂肪细胞的多能干细胞。
Stem Cells Transl Med. 2014 Feb;3(2):161-71. doi: 10.5966/sctm.2013-0107. Epub 2014 Jan 6.
8
Dedifferentiation of committed epithelial cells into stem cells in vivo.体内定向上皮细胞去分化为干细胞。
Nature. 2013 Nov 14;503(7475):218-23. doi: 10.1038/nature12777. Epub 2013 Nov 6.
9
Differences in gene expression and cytokine release profiles highlight the heterogeneity of distinct subsets of adipose tissue-derived stem cells in the subcutaneous and visceral adipose tissue in humans.基因表达和细胞因子释放谱的差异突出了人类皮下和内脏脂肪组织中不同脂肪组织来源干细胞亚群的异质性。
PLoS One. 2013;8(3):e57892. doi: 10.1371/journal.pone.0057892. Epub 2013 Mar 5.
10
Role of extracellular matrix remodelling in adipose tissue pathophysiology: relevance in the development of obesity.细胞外基质重塑在脂肪组织病理生理学中的作用：在肥胖症发展中的相关性。
Histol Histopathol. 2012 Dec;27(12):1515-28. doi: 10.14670/HH-27.1515.