Bocian-Ostrzycka Katarzyna M, Grzeszczuk Magdalena J, Banaś Anna M, Jagusztyn-Krynicka Elżbieta Katarzyna
Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
Appl Microbiol Biotechnol. 2017 May;101(10):3977-3989. doi: 10.1007/s00253-017-8291-8. Epub 2017 Apr 13.
The recent, rapid increase in bacterial antimicrobial resistance has become a major public health concern. One approach to generate new classes of antibacterials is targeting virulence rather than the viability of bacteria. Proteins of the Dsb system, which play a key role in the virulence of many pathogenic microorganisms, represent potential new drug targets. The first part of the article presents current knowledge of how the Dsb system impacts function of various protein secretion systems that influence the virulence of many pathogenic bacteria. Next, the review describes methods used to study the structure, biochemistry, and microbiology of the Dsb proteins and shows how these experiments broaden our knowledge about their function. The lessons gained from basic research have led to a specific search for inhibitors blocking the Dsb networks.
近期，细菌对抗菌药物的耐药性迅速增加，已成为一个主要的公共卫生问题。开发新型抗菌药物的一种方法是针对细菌的毒力而非其生存能力。Dsb系统的蛋白质在许多致病微生物的毒力中起关键作用，是潜在的新药物靶点。本文第一部分介绍了目前关于Dsb系统如何影响各种蛋白质分泌系统功能的知识，这些蛋白质分泌系统会影响许多致病细菌的毒力。接下来，该综述描述了用于研究Dsb蛋白质结构、生物化学和微生物学的方法，并展示了这些实验如何拓宽我们对其功能的认识。基础研究中获得的经验促使人们专门寻找阻断Dsb网络的抑制剂。
