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醛酮还原酶1（AKR1）通过清除种子老化过程中产生的具有细胞毒性的活性化合物来提高烟草和水稻种子的寿命。

Aldo-ketoreductase 1 (AKR1) improves seed longevity in tobacco and rice by detoxifying reactive cytotoxic compounds generated during ageing.

作者信息

Nisarga Kodadinne Narayana, Vemanna Ramu S, Kodekallu Chandrashekar Babitha, Rao Hanumantha, Vennapusa Amaranatha Reddy, Narasimaha Ashwini, Makarla Udayakumar, Basavaiah Mohan Raju

机构信息

Department of Crop Physiology, University of Agriculture Sciences, GKVK, Bengaluru, 560065, India.

出版信息

Rice (N Y). 2017 Dec;10(1):11. doi: 10.1186/s12284-017-0148-3. Epub 2017 Apr 13.

DOI:10.1186/s12284-017-0148-3
PMID:28409435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5391344/
Abstract

BACKGROUND

Maintenance of seed viability is an important factor for seedling vigour and plant establishment. Lipid peroxidation mediated reactive carbonyl compounds (RCC's) and non-enzymatic modifications of proteins through Maillard and Amadori products reduce seed viability and seedling vigour.

RESULTS

In this study, the relevance of RCCs on genotypic variation in rice seed viability and overexpression of an aldo-ketoreductase (AKR1) enzyme that detoxify cytotoxic compounds to improve seed viability and vigour was studied. Physiological and biochemical approaches were integrated to quantify the variation in seed viability and seedling vigour in rice genotypes after exposing to ageing treatment. AKR1 was overexpressed in a susceptible rice genotype and tobacco to study the relevance of reduced RCC's on seed viability and seedling vigour. The glycation and lipid peroxidation compounds accumulated after accelerated ageing treatments in rice genotypes. The accumulation of malondialdehyde, methyl glyoxal, Maillard and Amadori products affected the seed viability and germination as they showed a significant negative relationship. The transgenic rice and tobacco seeds expressing AKR1 showed lower levels of cytotoxic compounds and glycation products that resulted in improved seed viability and seedling vigour in rice and tobacco.

CONCLUSIONS

The study demonstrates that, reactive cytotoxic compounds affect the seed viability during storage. Detoxification of reactive cytotoxic compounds by Aldo-keto reductases is one of the mechanisms to improve the seed longevity during storage.

摘要

背景

种子活力的维持是幼苗活力和植株建立的重要因素。脂质过氧化介导的活性羰基化合物（RCCs）以及通过美拉德反应和阿马多里产物对蛋白质进行的非酶修饰会降低种子活力和幼苗活力。

结果

在本研究中，研究了RCCs对水稻种子活力基因型变异的相关性，以及一种能解毒细胞毒性化合物以提高种子活力和幼苗活力的醛酮还原酶（AKR1）的过表达情况。综合运用生理生化方法来量化水稻基因型在经过老化处理后种子活力和幼苗活力的变化。在一个易感水稻基因型和烟草中过表达AKR1，以研究降低RCCs对种子活力和幼苗活力的相关性。加速老化处理后，水稻基因型中糖基化和脂质过氧化化合物积累。丙二醛、甲基乙二醛、美拉德反应产物和阿马多里产物的积累影响了种子活力和萌发，因为它们呈现出显著的负相关关系。表达AKR1的转基因水稻和烟草种子显示出较低水平的细胞毒性化合物和糖基化产物，这导致水稻和烟草种子活力及幼苗活力得到提高。

结论

该研究表明，活性细胞毒性化合物在储存期间会影响种子活力。醛酮还原酶对活性细胞毒性化合物的解毒作用是提高储存期间种子寿命的机制之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/3f1ad550d988/12284_2017_148_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/2f429e863743/12284_2017_148_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/2c06e75eee85/12284_2017_148_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/ba9170f891de/12284_2017_148_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/1d63b37a5401/12284_2017_148_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/ccb1d3c0b9c6/12284_2017_148_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/3f1ad550d988/12284_2017_148_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/2f429e863743/12284_2017_148_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/2c06e75eee85/12284_2017_148_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/ba9170f891de/12284_2017_148_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/1d63b37a5401/12284_2017_148_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/ccb1d3c0b9c6/12284_2017_148_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d4/5391344/3f1ad550d988/12284_2017_148_Fig6_HTML.jpg

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