Alshaykh Mohammed S, Liao Chien-Sheng, Sandoval Oscar E, Gitzinger Gregory, Forget Nicolas, Leaird Daniel E, Cheng Ji-Xin, Weiner Andrew M
Opt Lett. 2017 Apr 15;42(8):1548-1551. doi: 10.1364/OL.42.001548.
Stimulated Raman scattering (SRS) is a powerful, label-free imaging technique that holds significant potential for medical imaging. To allow chemical specificity and minimize spectral distortion in the imaging of live species, a high-speed multiplex SRS imaging platform is needed. By combining a spectral focusing excitation technique with a rapid acousto-optic delay line, we demonstrate a hyperspectral SRS imaging platform capable of measuring a 3-dB spectral window of ∼200 cm within 12.8 μs with a scan rate of 30 KHz. We present hyperspectral images of a mixture of two different microsphere polymers as well as live fungal cells mixed with human blood.
受激拉曼散射（SRS）是一种强大的无标记成像技术，在医学成像方面具有巨大潜力。为了在活体成像中实现化学特异性并最小化光谱失真，需要一个高速多路复用SRS成像平台。通过将光谱聚焦激发技术与快速声光延迟线相结合，我们展示了一个高光谱SRS成像平台，该平台能够在12.8微秒内以30千赫兹的扫描速率测量约200厘米的3分贝光谱窗口。我们展示了两种不同微球聚合物混合物以及与人类血液混合的活真菌细胞的高光谱图像。
