Roth Richard H, Zhang Yong, Huganir Richard L
Solomon H. Snyder Department of Neuroscience, Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
Neuroscience Research Institute, Key Laboratory for Neuroscience, Ministry of Education/National Health and Family Planning Commission, Peking University, Beijing 100191, China. PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China.
Curr Opin Neurobiol. 2017 Aug;45:51-58. doi: 10.1016/j.conb.2017.03.008. Epub 2017 Apr 12.
Modulation of synaptic strength through trafficking of AMPA receptors is a fundamental mechanism underlying synaptic plasticity and has been shown to be an important process in higher brain functions such as learning and memory. Many studies have used live time-lapse imaging of fluorescently tagged AMPA receptors to directly monitor their membrane trafficking in the basal state as well as during synaptic plasticity. While most of these studies are performed in vitro using neuronal cell cultures, in the past years technological advances have enabled the imaging of synaptic proteins in vivo in intact organisms. This has allowed for visualization of synaptic plasticity on a molecular level in living and behaving animals. Here, we discuss key studies and approaches using dynamic imaging to visualize AMPA receptor trafficking in vitro as well as imaging synaptic proteins, including AMPA receptors, in vivo.
通过AMPA受体的转运来调节突触强度是突触可塑性的一个基本机制，并且已被证明是诸如学习和记忆等高等脑功能中的一个重要过程。许多研究使用荧光标记的AMPA受体的实时延时成像来直接监测其在基础状态以及突触可塑性期间的膜转运。虽然这些研究大多是在体外使用神经元细胞培养物进行的，但在过去几年中，技术进步使得在完整生物体中对体内突触蛋白进行成像成为可能。这使得在活体和行为动物中从分子水平可视化突触可塑性成为可能。在这里，我们讨论使用动态成像在体外可视化AMPA受体转运以及在体内成像包括AMPA受体在内的突触蛋白的关键研究和方法。
