Schickedanz J, Philipson L, Ansorge W, Pepperkok R, Klein R, Koszinowski U H
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
J Virol. 1988 Sep;62(9):3341-7. doi: 10.1128/JVI.62.9.3341-3347.1988.
The immediate-early (IE) genes of murine cytomegalovirus (MCMV) are expressed in the absence of prior viral protein synthesis and regulate the transcription of MCMV early genes. The effect of MCMV IE genes on growth induction was studied. Different plasmids containing MCMV IE genes were microinjected into arrested NIH 3T3 mouse fibroblasts. Plasmids containing the ieI gene coding for the 89,000-Mr major IE protein pp89 were found to stimulate the expression of the c-fos protooncogene. Synthesis of pp89 and its transport to the nucleus appeared to be required for c-fos expression. DNA synthesis occurred in cells that were injected with MCMV IE genes and in neighboring cells that were not injected. The results suggest that the phosphoprotein pp89 stimulates cells to enter the cell cycle.
鼠巨细胞病毒（MCMV）的立即早期（IE）基因在没有先前病毒蛋白合成的情况下表达，并调节MCMV早期基因的转录。研究了MCMV IE基因对生长诱导的影响。将含有MCMV IE基因的不同质粒显微注射到停滞的NIH 3T3小鼠成纤维细胞中。发现含有编码89,000-Mr主要IE蛋白pp89的ieI基因的质粒可刺激原癌基因c-fos的表达。pp89的合成及其向细胞核的转运似乎是c-fos表达所必需的。DNA合成发生在注射了MCMV IE基因的细胞以及未注射的邻近细胞中。结果表明，磷蛋白pp89刺激细胞进入细胞周期。
