Koszinowski U H, Reddehase M J, Keil G M, Schickedanz J
J Virol. 1987 Jun;61(6):2054-8. doi: 10.1128/JVI.61.6.2054-2058.1987.
To confirm that immediate-early (IE) genes of murine cytomegalovirus (MCMV) give rise to antigens recognized by specific cytolytic T lymphocytes (CTL), a 10.8-kilobase fragment of MCMV DNA which is abundantly transcribed at IE times was transfected into L cells expressing the Ld class I major histocompatibility glycoprotein. The viral genome fragment contains sequences of the three IE transcription units of MCMV: ie1, ie2, and ie3. In the transfected cell lines, only the predominant 2.75-kilobase transcript of ie1 and its translation product pp89 could be detected. The transfectants were analyzed for membrane expression of an IE antigen by employing clone IE1, an IE-specific CTL clone, as the probe. Only cells that expressed both the MCMV IE gene(s) and the Ld gene were recognized by the CTL clone.
为了证实小鼠巨细胞病毒（MCMV）的立即早期（IE）基因能产生可被特异性细胞溶解T淋巴细胞（CTL）识别的抗原，将一段在IE期大量转录的10.8千碱基的MCMV DNA片段转染到表达Ld I类主要组织相容性糖蛋白的L细胞中。该病毒基因组片段包含MCMV的三个IE转录单位的序列：ie1、ie2和ie3。在转染的细胞系中，只能检测到ie1的主要2.75千碱基转录本及其翻译产物pp89。通过使用克隆IE1（一种IE特异性CTL克隆）作为探针，分析转染细胞中IE抗原的膜表达。只有同时表达MCMV IE基因和Ld基因的细胞才能被CTL克隆识别。
