Tomecka Ewelina, Wojasinski Michal, Jastrzebska Elzbieta, Chudy Michal, Ciach Tomasz, Brzozka Zbigniew
Department of Microbioanalytics, Institute of Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
Department of Biotechnology and Bioprocess Engineering, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:305-316. doi: 10.1016/j.msec.2017.02.055. Epub 2017 Feb 15.
This paper presents a comparison and evaluation of cardiac cell proliferation on poly(l-lactic acid) (PLLA) and polyurethane (PU) nanofibrous mats fabricated by solution blow spinning (SBS). Three different cardiac cell lines: rat cardiomyoblasts (H9C2 line), human (HCM) and rat cardiomyocytes (RCM) were used for experiments. Cell morphology, orientation and proliferation were investigated on non-modified and protein-modified (fibronectin, collagen, gelatin, laminin, poly-l-lysine) surfaces of both types of nanofibers. Obtained results of cell culture on nanofibers surfaces were compared to the results of cell culture on polystyrene (PS) surfaces modified in the same way. The results indicated that in most cases polymeric nanofibers (PLLA and PU) are better substrates for cardiac cell culture than PS surfaces. All types of investigated cells, cultured on nanofibers (PLLA and PU), had more elongated shape than cells cultured on PS surfaces. Moreover, cells were arranged in parallel to each other, according to fibers orientation. Additionally, it was shown that the protein modifications of investigated surfaces influenced on cell proliferation. Therefore, we suggest that the cardiac cell culture on nanofibrous mats fabricated by SBS could be more advanced experimental in vitro model for studies on the effect of various cardiac drugs than traditional culture on PS surface.
本文介绍了通过溶液吹纺（SBS）制备的聚（L-乳酸）（PLLA）和聚氨酯（PU）纳米纤维垫上心脏细胞增殖的比较和评估。使用三种不同的心脏细胞系：大鼠心肌母细胞（H9C2系）、人（HCM）和大鼠心肌细胞（RCM）进行实验。在两种类型纳米纤维的未改性和蛋白质改性（纤连蛋白、胶原蛋白、明胶、层粘连蛋白、聚-L-赖氨酸）表面上研究细胞形态、取向和增殖。将在纳米纤维表面上获得的细胞培养结果与以相同方式改性的聚苯乙烯（PS）表面上的细胞培养结果进行比较。结果表明，在大多数情况下，聚合物纳米纤维（PLLA和PU）比PS表面更适合用于心脏细胞培养。在纳米纤维（PLLA和PU）上培养的所有类型的研究细胞，其形状比在PS表面上培养的细胞更细长。此外，细胞根据纤维取向彼此平行排列。另外，研究表明所研究表面的蛋白质改性会影响细胞增殖。因此，我们认为与传统的在PS表面培养相比，通过SBS制备的纳米纤维垫上进行心脏细胞培养可能是用于研究各种心脏药物作用的更先进的体外实验模型。
