Ain Qurat Ul, Khan Ahmad Nawaz, Nabavinia Mahboubeh, Mujahid Mohammad
Department of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, H-12, Islamabad, Pakistan.
Department of Materials Engineering, School of Chemical and Materials Engineering, National University of Sciences and Technology, H-12, Islamabad, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:807-815. doi: 10.1016/j.msec.2017.02.117. Epub 2017 Feb 24.
The bioactivity and mechanical properties of hybrid composites of hydroxyapatite (HA) in cyclic olefinic copolymer (COC) also known commercially as TOPAS are investigated, first time, for regeneration and repair of the bone tissues. HA is synthesized to obtain the spherically shaped nanoparticles in the size range of 60±20nm. Various concentrations of HA ranging from 1 to 30wt% are dispersed in TOPAS using sodium dodecyl sulfate (SDS) coupling agent for better dispersion and interaction of hydrophilic HA with hydrophobic TOPAS. Scanning electron microscope shows the uniform dispersion of HA≤10wt% in TOPAS and at higher concentrations >10wt%, agglomeration occurs in the hybrid composites. Tunable mechanical properties are achieved as the compressive modulus and strength are increased around 140% from 6.4 to 15.3MPa and 185% from 0.26 to 0.74MPa, respectively. Such increase in the mechanical properties of TOPAS is attributed to the anchoring of the polymer chains in the vicinity of HA nanoparticles owing to better dispersion and interfacial interactions. In comparison to neat TOPAS, hybrid composites of TOPAS/HA promoted the cell adhesion and proliferation significantly. The cell density and proliferation of TOPAS/HA hybrid composites is enhanced 9 and 3 folds, respectively, after 1day culturing in preosteoblasts cells. Moreover, the morphology of cells changed from spherical to flattened spread morphology demonstrating clearly the migration of the cells for the formation of interconnected cellular network. Additionally, very few dead cells are found in hybrid composites showing their cytocompatibility. Overall, the hybrid composites of TOPAS/HA exhibited superior strength and stiffness along with enhanced cytocompatibility for bone tissue engineering applications.
首次对环烯烃共聚物（COC，商品名为TOPAS）中羟基磷灰石（HA）杂化复合材料的生物活性和力学性能进行了研究，以用于骨组织的再生和修复。合成HA以获得尺寸范围为60±20nm的球形纳米颗粒。使用十二烷基硫酸钠（SDS）偶联剂将1至30wt%的各种浓度的HA分散在TOPAS中，以使亲水性HA与疏水性TOPAS更好地分散和相互作用。扫描电子显微镜显示HA≤10wt%在TOPAS中均匀分散，而在浓度>10wt%时，杂化复合材料中会发生团聚。实现了可调的力学性能，压缩模量和强度分别从6.4MPa增加到15.3MPa，提高了约140%，从0.26MPa增加到0.74MPa，提高了185%。TOPAS力学性能的这种增加归因于聚合物链由于更好的分散和界面相互作用而锚定在HA纳米颗粒附近。与纯TOPAS相比，TOPAS/HA杂化复合材料显著促进了细胞粘附和增殖。在成骨前体细胞中培养1天后，TOPAS/HA杂化复合材料的细胞密度和增殖分别提高了9倍和3倍。此外，细胞形态从球形变为扁平铺展形态，清楚地表明细胞迁移以形成相互连接的细胞网络。此外，在杂化复合材料中发现的死细胞很少，表明它们具有细胞相容性。总体而言，TOPAS/HA杂化复合材料在骨组织工程应用中表现出优异的强度和刚度以及增强的细胞相容性。
