Poirel Laurent, Kieffer Nicolas, Nordmann Patrice
Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland
French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland.
Antimicrob Agents Chemother. 2017 Jun 27;61(7). doi: 10.1128/AAC.00127-17. Print 2017 Jul.
The plasmid-mediated gene encodes a phosphoethanolamine transferase that confers resistance to polymyxins. The gene is associated with insertion sequence IS (IS family). mobilization assays demonstrated the functionality of the composite transposon structure IS-IS Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the gene by transposition.
质粒介导的基因编码一种磷酸乙醇胺转移酶，该酶赋予对多粘菌素的抗性。该基因与插入序列IS（IS家族）相关。移动分析证明了复合转座子结构IS-IS的功能。转座产生了一个2 bp的重复，并且发生在富含AT的DNA区域。这是首次证明该基因通过转座实现移动性的报告。
