Department of Medical Microbiology, Medical University of Warsaw, 5 Chałubiński Street, 02-004, Warsaw, Poland.
Data Analytics Unit, bioMérieux, La Balme-les-Grottes, France.
Eur J Clin Microbiol Infect Dis. 2017 Sep;36(9):1661-1664. doi: 10.1007/s10096-017-2980-1. Epub 2017 Apr 18.
Clostridium difficile is the cause of the nosocomial C. difficile infection (CDI). The conventional antibiotics used in CDI therapy are often unsuccessful, and recurrent infections may occur. Biofilm formation by C. difficile is associated with chronic or recurrent infections; biofilms may contribute to virulence and impaired antimicrobial efficacy. Manuka honey, derived from the Manuka tree (Leptospermum scoparium), is known to exhibit antimicrobial properties that are associated with its significant content of methylglyoxal, a natural antibiotic. The aim of the present study was to determine the antimicrobial effect of Manuka honey on clinical C. difficile strains belonging to four prominent polymerase chain reaction (PCR) ribotypes (RTs) (RT017, RT023, RT027 and RT046) and on their biofilm formation in vitro. Minimal inhibitory and bactericidal concentrations (MICs and MBCs, respectively) were determined using the broth dilution method. The biomass of the biofilm and the clearance of C. difficile biofilms by Manuka honey were determined using the crystal violet staining method. The MIC and MBC of Manuka honey for C. difficile strains were equal at 6.25% (v/v). PCR RT027 strains produced more biofilm in vitro than the other examined strains. Manuka honey effectively inhibited biofilm formation by C. difficile strains of different PCR RTs.
艰难梭菌是医院获得性艰难梭菌感染（CDI）的病因。CDI 治疗中使用的常规抗生素通常无效，可能会发生反复感染。艰难梭菌的生物膜形成与慢性或反复感染有关；生物膜可能有助于毒力和抗菌效果受损。麦卢卡蜂蜜源自麦卢卡树（Leptospermum scoparium），具有抗菌特性，这与其甲基乙二醛的高含量有关，甲基乙二醛是一种天然抗生素。本研究旨在确定麦卢卡蜂蜜对属于四个主要聚合酶链反应（PCR）核糖型（RT）（RT017、RT023、RT027 和 RT046）的临床艰难梭菌菌株的抗菌作用及其对体外生物膜形成的影响。使用肉汤稀释法测定最小抑菌和杀菌浓度（MIC 和 MBC）。使用结晶紫染色法测定生物膜的生物量和麦卢卡蜂蜜对艰难梭菌生物膜的清除率。麦卢卡蜂蜜对艰难梭菌菌株的 MIC 和 MBC 相等，均为 6.25%（v/v）。PCR RT027 菌株在体外产生的生物膜比其他检测到的菌株更多。麦卢卡蜂蜜可有效抑制不同 PCR RT 的艰难梭菌菌株的生物膜形成。
