Tola Esra Nur, Karatopuk Dilek Ulusoy, Koroglu Nadiye, Ergin Merve, Oral Hilmi Baha
Faculty of Medicine, Department of Gynecology and Obstetrics, In vitro Fertilization Unit, Suleyman Demirel University, Isparta, Turkey.
Faculty of Medicine, In vitro Fertilization Unit, Suleyman Demirel University, Isparta, Turkey.
J Assist Reprod Genet. 2017 Jun;34(6):811-816. doi: 10.1007/s10815-017-0913-7. Epub 2017 Apr 17.
Polycystic ovary syndrome (PCOS) is the most common cause of ovulatory dysfunction and female infertility. The etiopathogenetic mechanisms of PCOS have been studied for many years, although exact causes remain unclear. It has been demonstrated that proteoglycan degradation by a disintegrin-like metalloproteinase with thrombospondin type motifs-1 (ADAMTS-1) is essential for ovulation and fertilization. The objective of our study is to analyze the levels of ADAMTS-1 and aggrecan in the follicular fluid (FF) of PCOS patients compared with normal ovulatory women and to determine whether these markers could be a predictor of in vitro fertilization (IVF) success in PCOS patients.
Women with PCOS (n = 21) and normal ovulatory controls (n = 22) undergoing IVF treatment were recruited. ADAMTS-1 and aggrecan levels were analyzed with enzyme-linked immunosorbent assay (ELISA) and compared between PCOS and normal ovulatory controls. The predictor effect of ADAMTS-1 and aggrecan on fertilization rate and implantation was evaluated.
FF ADAMTS-1 and aggrecan levels increased in women with PCOS compared to controls. Elevated ADAMTS-1 levels but not aggrecan were related to increased implantation in PCOS.
Our study demonstrated that altered levels of ADAMTS-1 and aggrecan may have a partial role in the etiopathogenesis of PCOS, and ADAMTS-1 could be a predictive marker for implantation success in PCOS patients.
多囊卵巢综合征（PCOS）是排卵功能障碍和女性不孕的最常见原因。尽管确切病因尚不清楚，但PCOS的发病机制已研究多年。已证明具有血小板反应蛋白基序的解整合素样金属蛋白酶-1（ADAMTS-1）介导的蛋白聚糖降解对排卵和受精至关重要。我们研究的目的是分析PCOS患者与正常排卵女性卵泡液（FF）中ADAMTS-1和聚集蛋白聚糖的水平，并确定这些标志物是否可作为PCOS患者体外受精（IVF）成功的预测指标。
招募接受IVF治疗的PCOS患者（n = 21）和正常排卵对照者（n = 22）。采用酶联免疫吸附测定（ELISA）分析ADAMTS-1和聚集蛋白聚糖水平，并在PCOS患者和正常排卵对照者之间进行比较。评估ADAMTS-1和聚集蛋白聚糖对受精率和着床的预测作用。
与对照组相比，PCOS患者的FF中ADAMTS-1和聚集蛋白聚糖水平升高。PCOS患者中，ADAMTS-1水平升高与着床增加有关，而聚集蛋白聚糖水平升高与着床增加无关。
我们的研究表明，ADAMTS-1和聚集蛋白聚糖水平的改变可能在PCOS的发病机制中起部分作用，并且ADAMTS-1可能是PCOS患者着床成功的预测标志物。
