Kamal Abu Hena Mostafa, Rashid Hamid, Sakata Katsumi, Komatsu Setsuko
National Institute of Crop Science, NARO, Tsukuba 305-8518, Japan.
Mohammad Ali Jinnah University, Islamabad, Pakistan.
J Proteomics. 2015 Jan 1;112:1-13. doi: 10.1016/j.jprot.2014.08.014. Epub 2014 Sep 6.
Flooding stress causes growth inhibition and ultimately death in most crop species by limiting of energy production. To better understand plant responses to flooding stress, here, flooding-responsive proteins in the cotyledons of soybean were identified using a gel-free quantitative proteomic approach. One hundred forty six proteins were commonly observed in both control and flooding-stressed plants, and 19 were identified under only flooding stress conditions. The main functional categories were protein and development-related proteins. Protein-protein interaction analysis revealed that zincin-like metalloprotease and cupin family proteins were found to highly interact with other proteins under flooding stress. Plant stearoyl acyl-carrier protein, ascorbate peroxidase 1, and secretion-associated RAS superfamily 2 were down-regulated, whereas ferretin 1 was up-regulated at the transcription level. Notably, the levels of all corresponding proteins were decreased, indicating that mRNA translation to proteins is impaired under flooding conditions. Decreased levels of ferritin may lead to a strong deregulation of the expression of several metal transporter genes and over-accumulation of iron, which led to increased levels of reactive oxygen species, resulting to detoxification of these reactive species. Taken together, these results suggest that ferritin might have an essential role in protecting plant cells against oxidative damage under flooding conditions.
This study reported the comparative proteomic analysis of cotyledon of soybean plants between non-flooding and flooding conditions using the gel-free quantitative techniques. Mass spectrometry analysis of the proteins from cotyledon resulted in the identification of a total of 165 proteins under flooding stress. These proteins were assigned to different functional categories, such as protein, development, stress, redox, and glycolysis. Therefore, this study provides not only the comparative proteomic analysis but also the molecular mechanism underlying the flooding responsive protein functions in the cotyledon.
淹水胁迫通过限制能量产生导致大多数作物品种生长受抑制并最终死亡。为了更好地理解植物对淹水胁迫的反应，在此，采用无胶定量蛋白质组学方法鉴定了大豆子叶中的淹水响应蛋白。在对照植株和受淹水胁迫的植株中均普遍观察到146种蛋白质，仅在淹水胁迫条件下鉴定出19种蛋白质。主要功能类别为蛋白质和发育相关蛋白。蛋白质-蛋白质相互作用分析表明，在淹水胁迫下，锌指样金属蛋白酶和豆球蛋白家族蛋白与其他蛋白质高度相互作用。植物硬脂酰酰基载体蛋白、抗坏血酸过氧化物酶1和分泌相关RAS超家族2在转录水平上被下调，而铁蛋白1被上调。值得注意的是，所有相应蛋白质的水平均降低，表明在淹水条件下mRNA向蛋白质的翻译受到损害。铁蛋白水平降低可能导致几种金属转运蛋白基因表达的强烈失调以及铁的过度积累，从而导致活性氧水平升高，进而导致这些活性物质的解毒。综上所述，这些结果表明铁蛋白在淹水条件下保护植物细胞免受氧化损伤方面可能具有重要作用。
本研究报道了使用无胶定量技术对大豆植株在非淹水和淹水条件下子叶进行的比较蛋白质组学分析。对子叶蛋白质的质谱分析导致在淹水胁迫下共鉴定出165种蛋白质。这些蛋白质被归类为不同的功能类别，如蛋白质、发育、胁迫、氧化还原和糖酵解。因此，本研究不仅提供了比较蛋白质组学分析，还提供了子叶中淹水响应蛋白功能的分子机制。
