Boussy I A, Healy M J, Oakeshott J G, Kidwell M G
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson 85721.
Genetics. 1988 Aug;119(4):889-902. doi: 10.1093/genetics/119.4.889.
The latitudinal cline in P-M gonadal dysgenesis potential in eastern Australia has been shown to comprise three regions which are, from north to south respectively, P, Q, and M, with the P-to-Q and Q-to-M transitions occurring over relatively short distances. The P element complements of 30 lines from different regions of the cline were determined by molecular techniques. The total amount of P element-hybridizing DNA was high in all lines, and it did not correlate in any obvious way with the P-M phenotypes of individual lines. The number of potentially full-sized P elements per genome was high in lines from the P regions, but variable or low among lines from the Q and M regions, and thus declined overall from north to south. A particular P element deletion-derivative, the KP element, occurred in all the tested lines. The number of KP elements was low in lines from the P region, much higher in lines from the Q region, and highest among lines from the M region, thus forming a cline reciprocal to that of the full-sized P elements. Another transposable element, hobo, which has been described as causing dysgenic traits similar to those of P-M hybrid dysgenesis, was shown to be present in all lines and to vary among them in number, but not in any latitudinal pattern. The P-M cline in gonadal dysgenesis potential can be inferred to be based on underlying clinal patterns of genomic P element complements. P activity of a line was positively correlated with the number of full-sized P elements in the line, and negatively correlated with the number of KP elements. Among Q and M lines, regulatory ability was not correlated with numbers of KP elements.
在澳大利亚东部，P-M性腺发育不全潜力的纬度渐变已被证明由三个区域组成，从北到南分别为P、Q和M，P到Q以及Q到M的转变发生在相对较短的距离内。通过分子技术确定了来自渐变不同区域的30个品系的P元件互补情况。所有品系中与P元件杂交的DNA总量都很高，并且与各个品系的P-M表型没有任何明显的相关性。每个基因组中潜在的完整大小P元件数量在来自P区域的品系中很高，但在来自Q和M区域的品系中则可变或较低，因此总体上从北到南呈下降趋势。一种特定的P元件缺失衍生物，即KP元件，出现在所有测试品系中。KP元件的数量在来自P区域的品系中较低，在来自Q区域的品系中高得多，在来自M区域的品系中最高，因此形成了与完整大小P元件相反的渐变。另一种转座元件hobo，已被描述为会导致类似于P-M杂种发育不全的发育异常性状，结果表明它存在于所有品系中，且数量在品系间有所不同，但没有任何纬度模式。性腺发育不全潜力的P-M渐变可以推断是基于基因组P元件互补的潜在渐变模式。一个品系的P活性与该品系中完整大小P元件的数量呈正相关，与KP元件的数量呈负相关。在Q和M品系中，调控能力与KP元件的数量无关。
