Nichols R, Schneuwly S A, Dixon J E
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
J Biol Chem. 1988 Sep 5;263(25):12167-70.
Homologues to the cholecystokinin (CCK)-gastrin peptide family have been cloned from Drosophila. The CCK-like precursor found in Drosophila has been designated drosulfakinin (DSK). Genomic and cDNA clones corresponding to the Drosophila neuropeptide precursor encode for three putative peptides. The three peptides (DSK-0, Asn-Gln-Lys-Thr-Met-Ser-Phe-Gly; DSK-I, Phe-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-Gly; DSK-II, Gly-Gly-Asp-Asp-Gln-Phe-Asp-Asp-Tyr-Gly-His-Met-Arg-Phe-Gly) are flanked by prohormone processing sites and contain C-terminal glycyl residues, a potential amidation site. Two of the peptides, DSK-I and DSK-II, are homologous to CCK-gastrin peptides. Each of the two homologues include a CCK-gastrin-like C-terminal pentapeptide and a conserved sequence corresponding to the sulfated tyrosine in bioactive CCK. The third peptide encoded by the drosulfakinin precursor represents a novel peptide. In situ tissue hybridization indicates the presence of the transcript in the adult head. Chromosomal localization maps the gene to the third chromosome near 81F.
已从果蝇中克隆出与胆囊收缩素（CCK）-胃泌素肽家族同源的物质。在果蝇中发现的CCK样前体被命名为果蝇速激肽（DSK）。与果蝇神经肽前体对应的基因组和cDNA克隆编码三种假定的肽。这三种肽（DSK-0，天冬酰胺-谷氨酰胺-赖氨酸-苏氨酸-甲硫氨酸-丝氨酸-苯丙氨酸-甘氨酸；DSK-I，苯丙氨酸-天冬氨酸-天冬氨酸-酪氨酸-甘氨酸-组氨酸-甲硫氨酸-精氨酸-苯丙氨酸-甘氨酸；DSK-II，甘氨酸-甘氨酸-天冬氨酸-天冬氨酸-谷氨酰胺-苯丙氨酸-天冬氨酸-天冬氨酸-酪氨酸-甘氨酸-组氨酸-甲硫氨酸-精氨酸-苯丙氨酸-甘氨酸）两侧是激素原加工位点，并含有C末端甘氨酰残基，这是一个潜在的酰胺化位点。其中两种肽，DSK-I和DSK-II，与CCK-胃泌素肽同源。这两种同源物中的每一种都包括一个CCK-胃泌素样C末端五肽和一个与生物活性CCK中硫酸化酪氨酸相对应的保守序列。果蝇速激肽前体编码的第三种肽代表一种新肽。原位组织杂交表明该转录本在成年头部存在。染色体定位将该基因定位到靠近81F的第三条染色体上。
