Yang Taowei, Rao Zhiming, Zhang Xian, Xu Meijuan, Xu Zhenghong, Yang Shang-Tian
a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology , Jiangnan University , Wuxi , China.
b Jiangnan University (Rugao) Food Biotechnology Research Institute , Rugao , Jiangsu Province , China.
Crit Rev Biotechnol. 2017 Dec;37(8):990-1005. doi: 10.1080/07388551.2017.1299680. Epub 2017 Apr 20.
Acetoin and 2,3-butanediol (2,3-BD) have a large number of industrial applications. The production of acetoin and 2,3-BD has traditionally relied on oil supplies. Microbial production of acetoin and 2,3-BD will alleviate the dependence on oil. Acetoin and 2,3-BD are neighboring metabolites in the 2,3-BD metabolic pathway of bacteria. This review summarizes metabolic engineering strategies for improvement of microbial acetoin and 2,3-BD production. We also propose enhancements to current acetoin and 2,3-BD production strategies, by offering a metabolic engineering approach that is guided by systems biology and synthetic biology.
乙偶姻和2,3-丁二醇（2,3-BD）有大量的工业应用。传统上，乙偶姻和2,3-丁二醇的生产依赖于石油供应。通过微生物生产乙偶姻和2,3-丁二醇将减轻对石油的依赖。乙偶姻和2,3-丁二醇是细菌2,3-丁二醇代谢途径中的相邻代谢产物。本文综述了改善微生物生产乙偶姻和2,3-丁二醇的代谢工程策略。我们还通过提供一种以系统生物学和合成生物学为指导的代谢工程方法，对当前乙偶姻和2,3-丁二醇的生产策略提出改进建议。
