Department of Chemical and Biological Engineering, University of Ottawa, 161 Louis Pasteur Private, Ottawa, ON K1N 6N5, Canada.
Biotechnol Adv. 2019 Jul-Aug;37(4):569-578. doi: 10.1016/j.biotechadv.2018.03.019. Epub 2018 Mar 31.
As a significant platform chemical, 2, 3-butanediol (2, 3-BD) has found wide applications in industry. The success of microbial 2, 3-BD production was limited by the use of pathogenic microorganisms and low titer in engineered hosts. The utilization of cheaply available feedstock such as lignocellulose was another major challenge to achieve economic production of 2, 3-BD. To address those issues, engineering strategies including both genetic modifications and process optimization have been employed. In this review, we summarized the state-of-the-art progress in the biotechnological production of 2, 3-BD. Metabolic engineering and process engineering strategies were discussed.
作为一种重要的平台化学品，2,3-丁二醇（2,3-BD）在工业中有着广泛的应用。微生物 2,3-BD 的生产受到使用病原微生物和工程宿主中低产量的限制。利用廉价的原料如木质纤维素是实现 2,3-BD 经济生产的另一个主要挑战。为了解决这些问题，已经采用了包括遗传修饰和过程优化在内的工程策略。在这篇综述中，我们总结了生物技术生产 2,3-BD 的最新进展。讨论了代谢工程和过程工程策略。
