Graduate Program in Pharmaceutical Sciences, University of Vila Velha (UVV), Vila Velha, ES, Brazil.
Undergraduate Medicine, University of Vila Velha (UVV), Vila Velha, ES, Brazil.
Ultrason Sonochem. 2017 Jul;37:368-374. doi: 10.1016/j.ultsonch.2017.01.035. Epub 2017 Jan 26.
Achyrocline satureioides or Macela, has been largely used in traditional folk medicine in Brazil as an anti-inflammatory agent and to treat various digestive disorders. The aim of the present study was to evaluate the preventive action of the extracts of A. satureioides obtained by maceration and ultrasound-assisted extraction, quercetin and N-acetylcysteine against contrast-induced nephropathy in mice. The antioxidant activity, cytotoxicity and inhibition of nitric oxide (NO) production in macrophages were evaluated. Also, chemical analyses of phenolic compounds, total flavonoids, and quercetin by LC-MS/MS present in various extracts of A. satureioides were performed. Thirty six mice were divided into six groups: control group (C), Contrast-Induced Nephropathy group (CIN), Group N-acetylcysteine 200mg/kg (NAC); Group quercetin 10mg/kg (Q), Group Macela 10mg/kg (M10), and Group Macela 50mg/kg (M50). The serum levels of urea and creatinine, advanced oxidation protein products (AOPP) and renal ultrastructure were evaluated by electron microscopy scanning. Ultrasound-assisted extraction improved the quality of extract (with 100% ethanol), since did not show toxicity to fibroblasts, and showed potent antioxidant activity and a high content of phenolic compounds, flavonoids, and quercetin, in addition to being able to reduce the production of NO in dose-dependent effect in macrophages. Results showed that animals treated with Macela extracts maintained normal levels of urea, creatinine, and AOPP, while preserving ultrastructure of the renal cells. The obtained results were more promising than NAC and Q groups in protecting against renal failure caused by CIN, showing that the plant can be a promising drug for preventing this disease.
旱莲草或 Macela，在巴西的传统民间医学中被广泛用作抗炎剂，并用于治疗各种消化疾病。本研究旨在评估浸渍法和超声辅助提取、槲皮素和 N-乙酰半胱氨酸获得的旱莲草提取物对小鼠造影剂肾病的预防作用。评估了抗氧化活性、细胞毒性和巨噬细胞中一氧化氮 (NO) 产生的抑制作用。此外，还通过 LC-MS/MS 对旱莲草各种提取物中的酚类化合物、总类黄酮和槲皮素进行了化学分析。将 36 只小鼠分为 6 组：对照组 (C)、造影剂肾病组 (CIN)、N-乙酰半胱氨酸 200mg/kg 组 (NAC)、槲皮素 10mg/kg 组 (Q)、旱莲草 10mg/kg 组 (M10) 和旱莲草 50mg/kg 组 (M50)。通过电子显微镜扫描评估血清尿素和肌酐、晚期氧化蛋白产物 (AOPP) 和肾脏超微结构。超声辅助提取提高了提取物的质量（使用 100%乙醇），因为它对成纤维细胞没有毒性，并且表现出强大的抗氧化活性和高含量的酚类化合物、类黄酮和槲皮素，此外还能够以剂量依赖的方式降低巨噬细胞中 NO 的产生。结果表明，用旱莲草提取物治疗的动物保持了尿素、肌酐和 AOPP 的正常水平，同时保持了肾脏细胞的超微结构。与 NAC 和 Q 组相比，旱莲草提取物在预防 CIN 引起的肾衰竭方面更有前景，表明该植物可能是预防这种疾病的有前途的药物。
