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应用于复合物的分子分型方法比较：测序、重复序列聚合酶链反应和多位点序列分型

A Comparison of Molecular Typing Methods Applied to complex: Sequencing, Rep-PCR, and MLST.

作者信息

Viau Roberto A, Kiedrowski Lee M, Kreiswirth Barry N, Adams Mark, Perez Federico, Marchaim Dror, Guerrero Dubert M, Kaye Keith S, Logan Latania K, Villegas Maria Virginia, Bonomo Robert A

机构信息

Medical and Research Services Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH.

North Dakota State University, Fargo, ND.

出版信息

Pathog Immun. 2017;2(1):23-33. doi: 10.20411/pai.v2i1.99. Epub 2017 Feb 1.

DOI:10.20411/pai.v2i1.99
PMID:28428984
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5394936/
Abstract

Molecular typing using repetitive sequenced-based PCR (rep-PCR) and sequencing were applied to a collection of diverse complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

摘要

使用基于重复序列的聚合酶链反应（rep-PCR）和测序技术对一系列不同的复杂分离株进行分子分型。为了确定参考实验室最实用的方法，我们分析了来自4个地理区域散发病例和暴发疫情中的71株复杂分离株。虽然rep-PCR的区分能力更强，但测序提供了一种更广泛、更客观的地理追踪方法，类似于多位点序列分型（MLST）。此外，我们认为与测序相比，MLST可能具有更高的区分能力，尽管rep-PCR仍然是本地暴发调查中区分能力最强的方法。此外，rep-PCR可以成为本地暴发调查的一种有效且廉价的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/2f060a6db4d1/pai-2-023-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/0e2018b7517e/pai-2-023-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/68b02db3ff01/pai-2-023-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/41cd30736c32/pai-2-023-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/2f060a6db4d1/pai-2-023-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/0e2018b7517e/pai-2-023-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/68b02db3ff01/pai-2-023-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/41cd30736c32/pai-2-023-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb93/6423641/2f060a6db4d1/pai-2-023-g004.jpg

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