Willwand K, Kaaden O R
Institute of Virology, Hannover Veterinary School, Federal Republic of Germany.
Virology. 1988 Sep;166(1):52-7. doi: 10.1016/0042-6822(88)90145-6.
The interaction between Aleutian disease virus (ADV) DNA and proteins isolated from ADV-infected cells or ADV virions, respectively, was examined. Proteins were separated on SDS-polyacrylamide gels, transferred to nitrocellulose, and probed with 32P-labeled restriction fragments of replicative form (RF) DNA or with single-stranded virion DNA. The ADV capsid protein VP1 was found to bind the 3'-terminal BamHI fragment of RF DNA extending from map units (m.u.) 0 to 15. No binding was observed with the fragment extending from m.u. 15 to 63 and only minor amounts of label were detected with the 5'-terminal EcoRV fragment extending from m.u. 63 to 100. With the latter fragment, small amounts of label were also detected bound to higher-molecular-weight proteins of about 100,000 Da. No binding of DNA to the ADV nonstructural protein NS1 or to the ADV capsid protein VP2 was detected. Additionally, single-stranded virion DNA was found to bind to VP1.
分别检测了阿留申病病毒（ADV）DNA与从ADV感染细胞或ADV病毒粒子中分离出的蛋白质之间的相互作用。蛋白质在SDS-聚丙烯酰胺凝胶上分离，转移至硝酸纤维素膜上，并用复制型（RF）DNA的32P标记限制性片段或单链病毒粒子DNA进行检测。发现ADV衣壳蛋白VP1与RF DNA从图谱单位（m.u.）0至15延伸的3'-末端BamHI片段结合。未观察到与从m.u. 15至63延伸的片段结合，并且仅检测到少量放射性标记与从m.u. 63至100延伸的5'-末端EcoRV片段结合。对于后一片段，还检测到少量放射性标记与约100,000 Da的高分子量蛋白质结合。未检测到DNA与ADV非结构蛋白NS1或ADV衣壳蛋白VP2结合。此外，发现单链病毒粒子DNA与VP1结合。
