[Analysis of antibody formation to the vaccinia virus in human subjects and rabbits in response to the administration of a recombinant vaccinia-hepatitis B vaccine].

Immune blotting analysis was used to study antibody production to virion proteins of vaccinia virus (VV) in response to inoculation of human volunteers and rabbits with VV (LIVP strain) and its genetic engineering recombinant variants carrying a gene of hepatitis B virus surface antigen (LIOGEN-HB/C2-TK-) and, plus to that, the TK gene of herpes simplex virus (LIOGEN-HB/C2-TK+). In the blood of human subjects vaccinated 10-15 years earlier antibodies to many virion proteins were demonstrated: not only to surface 42K, 35K, 11K, but also to nucleoid proteins 135K, 88K, 62K, 60K, 26K. Vaccination with genetic engineering viruses results in stimulation of synthesis of antibodies to VV virion proteins. No differences in the antigenic properties of recombinant viruses were found. The results obtained in model experiments in rabbits verified those obtained in human volunteers.