上游序列对水痘带状疱疹病毒基因27体外翻译的调控
Regulation of varicella zoster virus gene 27 translation in vitro by upstream sequences.
作者信息
Mahalingam R, Wellish M, Cabirac G, Gilden D, Vafai A
机构信息
Department of Neurology, University of Colorado Health Sciences Center, Denver 80262.
出版信息
Virus Res. 1988 May;10(2-3):193-204. doi: 10.1016/0168-1702(88)90015-9.
Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA transcribed from these open reading frames showed prominent expression of gene 27. Four different subclones were constructed to contain gene 27 with and without 100 base pairs (bp) of upstream sequences. Translation of RNA from these constructs using wheat germ extract or rabbit reticulocyte lysate indicated that the sequences upstream from the predicted initiation codon (AUG) of gene 27 downregulated the expression of this gene at the level of translation and that the predicted AUG within gene 27 was preferentially used.
Northern印迹分析显示在受感染细胞中存在水痘带状疱疹病毒（VZV）基因27转录本。对位于VZV基因组独特长片段中且包含重叠基因26和27的Sal I-G DNA片段，在体外转录-翻译系统中进行了分析。从这些开放阅读框转录的RNA的翻译显示基因27有显著表达。构建了四个不同的亚克隆，分别包含有和没有100个碱基对（bp）上游序列的基因27。使用麦胚提取物或兔网织红细胞裂解物对这些构建体的RNA进行翻译，结果表明基因27预测起始密码子（AUG）上游的序列在翻译水平下调了该基因的表达，并且基因27内预测的AUG被优先使用。
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