Gilden D H, Shtram Y, Friedmann A, Wellish M, Devlin M, Cohen A, Fraser N, Becker Y
J Virol Methods. 1982 May;4(4-5):263-75. doi: 10.1016/0166-0934(82)90073-8.
Varicella-zoster virus (VZV) DNA was extracted from infected cells with 0.25% Triton X-100-0.2 M NaCl and purified by isopycnic centrifugation in CsCl. In each of eight experiments, 1.8-9.8 micrograms VZV DNA was obtained from 107 infected cells. The VZV DNA obtained by this procedure had a molecular weight of 88-100 x 106 as determined by sucrose gradient sedimentation and electron microscopy, and cleavage patterns after digestion with four restriction enzymes that corresponded to patterns previously described with six strains of VZV; the pattern of BamHI-cleaved Triton-NaCl-extracted VZV DNA was identical to the pattern seen after DNA extraction from virions. These studies expand the usefulness of Triton X-100-NaCl for extraction of large molecular weight viral DNA from a system where considerable cell-free virus is produced (Pignatti et al., 1979, Virology 93, 260) to a system known for its marked cell association.
用含0.25% Triton X-100的0.2M NaCl从感染细胞中提取水痘带状疱疹病毒（VZV）DNA，并通过在CsCl中进行等密度离心进行纯化。在8次实验的每一次中，从107个感染细胞中获得了1.8 - 9.8微克的VZV DNA。通过该方法获得的VZV DNA的分子量经蔗糖梯度沉降和电子显微镜测定为88 - 100×106，在用四种限制性内切酶消化后的切割模式与先前用六株VZV描述的模式相对应；BamHI切割的Triton-NaCl提取的VZV DNA模式与从病毒粒子中提取DNA后观察到的模式相同。这些研究将Triton X-100-NaCl从用于从产生大量无细胞病毒的系统（Pignatti等人，1979年，《病毒学》93卷，260页）中提取大分子量病毒DNA的有用性扩展到了一个以显著细胞关联而闻名的系统。
