单纯疱疹病毒1型在非洲绿猴肾细胞中成熟过程的定量研究。

Quantitative studies on the maturation process of herpes simplex virus type 1 in Vero cells.

作者信息

Koyama A H, Uchida T

机构信息

Department of Virology, School of Medicine, University of Tokushima, Japan.

出版信息

Virus Res. 1988 May;10(2-3):281-5. doi: 10.1016/0168-1702(88)90023-8.

DOI:10.1016/0168-1702(88)90023-8

PMID:2842975

Abstract

We examined the time course of viral DNA synthesis, the formation and envelopment of viral nucleocapsids, and the formation of infectious progeny virus in Vero cells infected with herpes simplex virus type 1 (HSV-1). The results showed that the formation of nucleocapsids coincided with the appearance of enveloped particles as well as of infectious progeny virus, although the synthesis of viral DNA took place approximately 2 h prior to the beginning of nucleocapsid formation. These results indicate that the rate-limiting step in the virogenesis of HSV-1-infected cells is the encapsidation of viral DNA and that the enveloped virus is formed immediately after the formation of nucleocapsids and is infectious without any further processing of virion constituents.

摘要

我们研究了感染1型单纯疱疹病毒（HSV-1）的非洲绿猴肾细胞（Vero细胞）中病毒DNA合成的时间进程、病毒核衣壳的形成与包裹以及感染性子代病毒的形成。结果显示，核衣壳的形成与包膜颗粒以及感染性子代病毒的出现同时发生，尽管病毒DNA的合成在核衣壳形成开始前约2小时就已进行。这些结果表明，HSV-1感染细胞病毒发生过程中的限速步骤是病毒DNA的衣壳化，并且包膜病毒在核衣壳形成后立即形成，无需对病毒粒子成分进行任何进一步加工即具有感染性。