Bucsky P, Hampl W, Frickhofen N, Gaedicke G
Dept. of Virology (Microbiology) I, University of Ulm, FRG.
Acta Morphol Hung. 1987;35(3-4):175-81.
The origin of Reed-Sternberg cell is still unresolved. It is known that in the cultures of mononuclear blood cells stimulated by pokeweed mitogen, Reed-Sternberg like cells occur. Most of these cells have the phenotype of T lymphocytes. To investigate the fusion theory, as one of the possibilities for the origin of Reed-Sternberg cells, we have infected mononuclear blood cells from normal donors by Epstein-Barr virus as a T cell independent B lymphocyte stimulator. In these cell cultures, large multinucleated cells resembling Reed-Sternberg cells were found. These cells usually have the phenotype of B lymphocytes, some of them, however, also have markers of monocytes/macrophages. The possibility of an in vitro fusion between B lymphocytes and monocytes induced by Epstein-Barr virus may be assumed. As to the origin of Reed-Sternberg cells, an in vivo fusion between B-lymphocyte and monocyte/macrophage is proposed.
里德-施特恩伯格细胞的起源仍未解决。已知在由商陆有丝分裂原刺激的单核血细胞培养物中会出现里德-施特恩伯格样细胞。这些细胞中的大多数具有T淋巴细胞的表型。为了研究融合理论，作为里德-施特恩伯格细胞起源的可能性之一，我们用爱泼斯坦-巴尔病毒作为一种不依赖T细胞的B淋巴细胞刺激剂感染了正常供体的单核血细胞。在这些细胞培养物中，发现了类似于里德-施特恩伯格细胞的大型多核细胞。这些细胞通常具有B淋巴细胞的表型，然而，其中一些也具有单核细胞/巨噬细胞的标志物。可以推测爱泼斯坦-巴尔病毒诱导B淋巴细胞和单核细胞在体外融合的可能性。关于里德-施特恩伯格细胞的起源，有人提出B淋巴细胞与单核细胞/巨噬细胞在体内融合。
