1Department of Pathology, Botucatu Medical School, UNESP, Universidade Estadual Paulista, São Paulo, Brazil; 2Department of Basic Pathology, Setor de Ciências Biológicas, UFPR, Universidade Federal do Paraná, Curitiba, Brazil; and 3The University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil.
J Low Genit Tract Dis. 2017 Jul;21(3):189-192. doi: 10.1097/LGT.0000000000000315.
This study included women attending primary health care units in Botucatu, São Paulo, Brazil, to assess the cervicovaginal levels of human β-defensin (hBD) 1, 2, 3, and 4 during Chlamydia trachomatis infection.
Cervicovaginal samples were collected for Pap testing and assessing the presence of infection by C. trachomatis, human papillomavirus, Neisseria gonorrhoeae, and Trichomonas vaginalis. Vaginal smears were taken to evaluate local microbiota. Human β-defensin levels were determined using enzyme-linked immunosorbent assay in cervicovaginal fluid samples. Seventy-four women with normal vaginal microbiota and no evidence of infection were included in hBD quantification assays; 37 tested positive for C. trachomatis and 37 were negative. Statistical analysis was performed using Mann-Whitney U test.
Women positive for C. trachomatis had significantly lower cervicovaginal hBD-1, hBD-2, and hBD-3 compared with those who tested negative (hBD-1: 0 pg/mL [0-2.1] vs 1.6 pg/mL [0-2.4], p < .0001; hBD-2: 0 pg/mL [0-3.9] vs 0.61 pg/mL [0-8.9], p = .0097; and hBD-3: 0 pg/mL [0-4.3] vs 0.28 pg/mL [0-8.4], p = .0076). Human β-defensin 4 was not detected.
Lower levels of hBD-1, hBD-2, and hBD-3 in cervicovaginal fluid were detected in the presence of C. trachomatis infection.
本研究纳入了巴西圣保罗博图卡图市初级保健单位就诊的女性，旨在评估沙眼衣原体感染时宫颈阴道中人β防御素（hBD）1、2、3 和 4 的水平。
采集宫颈阴道标本进行巴氏涂片检查，并评估沙眼衣原体、人乳头瘤病毒、淋病奈瑟菌和阴道毛滴虫感染情况。取阴道涂片评估局部微生物群。采用酶联免疫吸附试验测定宫颈阴道液样本中的 hBD 水平。74 例阴道微生物群正常且无感染证据的女性纳入 hBD 定量检测，其中 37 例沙眼衣原体阳性，37 例为阴性。采用 Mann-Whitney U 检验进行统计分析。
沙眼衣原体阳性女性的宫颈阴道 hBD-1、hBD-2 和 hBD-3 水平明显低于阴性女性（hBD-1：0 pg/mL [0-2.1] 与 1.6 pg/mL [0-2.4]，p <.0001；hBD-2：0 pg/mL [0-3.9] 与 0.61 pg/mL [0-8.9]，p =.0097；hBD-3：0 pg/mL [0-4.3] 与 0.28 pg/mL [0-8.4]，p =.0076）。未检测到 hBD-4。
沙眼衣原体感染时宫颈阴道液中 hBD-1、hBD-2 和 hBD-3 水平降低。
