Marconi Camila, Santos-Greatti Mariana M V, Parada Cristina M G L, Pontes Anagloria, Pontes Ana G, Giraldo Paulo C, Donders Gilbert G G, da Silva Márcia Guimarães
Departments of 1Pathology, 2Nursing, and 3Gynecology and Obstetrics, Botucatu Medical School, UNESP - Univ Estadual Paulista; 4Department of Gynecology and Obstetrics, Faculdade de Ciências Médicas, Campinas University, Campinas, Brazil; and 5University Hospital Gasthuisberg, Leuven, Belgium.
J Low Genit Tract Dis. 2014 Jul;18(3):261-5. doi: 10.1097/LGT.0000000000000003.
The purpose of this study was to assess the cervicovaginal levels of proinflammatory cytokines in women with Chlamydia trachomatis (CT) infection in the presence of bacterial vaginosis (BV) and normal flora and to compare with those negative for CT.
In this cross-sectional study, nonpregnant women were enrolled at 2 outpatient clinics and at 1 primary medical care unit in São Paulo State, Brazil. Cervicovaginal samples from 256 women with BV, of which 68 (26.6%) had concomitant CT infection and 188 (73.4%) were CT-negative, were measured for interleukin-1β (IL-1β), IL-6, and IL-8 by enzyme-linked immunosorbent assay. A matching number of samples from women with normal flora, CT-positive (n = 68) and negative (n = 188), were evaluated as control. Cytokine levels were compared by Mann-Whitney test and differences were considered significant at p < .05.
In CT-negative women, IL-1β was increased in BV (p < .001) when compared to normal flora, while the levels of IL-6 and IL8 were unchanged. The presence of CT infection was not associated with differences on cytokine levels in women with normal flora. However, women with BV had higher levels of IL-1β (p = .02), IL-6 (p = .02), and IL-8 (p = .03) in the presence of CT when compared to those who tested negative for CT.
Detection of endocervical CT is associated with increased cervicovaginal IL-1β, IL-6, and IL-8 levels in women with concomitant BV but not in those with normal flora.
本研究旨在评估沙眼衣原体（CT）感染合并细菌性阴道病（BV）及正常菌群的女性宫颈阴道内促炎细胞因子水平，并与CT阴性女性进行比较。
在这项横断面研究中，非妊娠女性来自巴西圣保罗州的2个门诊诊所和1个基层医疗单位。对256例患有BV的女性的宫颈阴道样本进行检测，其中68例（26.6%）合并CT感染，188例（73.4%）CT阴性，采用酶联免疫吸附测定法检测白细胞介素-1β（IL-1β）、IL-6和IL-8。选取数量匹配的正常菌群女性样本，CT阳性（n = 68）和阴性（n = 188），作为对照进行评估。细胞因子水平采用曼-惠特尼检验进行比较，p <.05时差异具有统计学意义。
在CT阴性女性中，与正常菌群相比，BV患者的IL-1β水平升高（p <.001），而IL-6和IL-8水平无变化。CT感染的存在与正常菌群女性的细胞因子水平差异无关。然而，与CT检测阴性的女性相比，合并CT感染的BV女性的IL-1β（p =.02）、IL-6（p =.02）和IL-8（p =.03）水平更高。
宫颈管CT检测与合并BV的女性宫颈阴道IL-1β、IL-6和IL-8水平升高有关，但与正常菌群女性无关。
