Preston D R, Vasudevan T V, Bitton G, Farrah S R, Morel J L
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Appl Environ Microbiol. 1988 Jun;54(6):1325-9. doi: 10.1128/aem.54.6.1325-1329.1988.
Electronegative microporous filters composed of epoxyfiberglass (Filterite) were treated with cationic polymers to enhance their virus-adsorbing properties. This novel and inexpensive approach to microporous filter modification entails soaking filters in an aqueous solution of a cationic polymer such as polyethyleneimine (PEI) for 2 h at room temperature and then allowing the filters to air dry overnight on absorbent paper towels. PEI-treated filters were evaluated for coliphage (MS2, T2, and phi X174) and enterovirus (poliovirus type 1 and coxsackievirus type B5) adsorption from buffer at pH 3.5 to 9.0 and for indigenous coliphages from unchlorinated secondary effluent at ambient pH. Adsorbed viruses were recovered with 3% beef extract (pH 9). Several other cationic polymers were used to modify epoxyfiberglass filters and were evaluated for their ability to concentrate viruses from water. Zeta potentials of disrupted filter material indicated that electronegative epoxyfiberglass filters were made more electropositive when treated with cationic polymers. In general, epoxyfiberglass filters treated with cationic polymers were found to adsorb a greater percentage of coliphages and enteroviruses than were untreated filters.
由环氧玻璃纤维（Filterite）制成的带负电的微孔过滤器用阳离子聚合物进行处理，以增强其病毒吸附特性。这种新颖且廉价的微孔过滤器改性方法包括将过滤器在阳离子聚合物（如聚乙烯亚胺，PEI）的水溶液中于室温下浸泡2小时，然后让过滤器在吸水纸巾上自然风干过夜。对经PEI处理的过滤器进行了评估，以检测其在pH值为3.5至9.0的缓冲液中对大肠杆菌噬菌体（MS2、T2和φX174）和肠道病毒（1型脊髓灰质炎病毒和B5型柯萨奇病毒）的吸附情况，以及在环境pH值下对未氯化二级出水的本地大肠杆菌噬菌体的吸附情况。吸附的病毒用3%牛肉提取物（pH值为9）进行回收。还使用了其他几种阳离子聚合物对环氧玻璃纤维过滤器进行改性，并评估了它们从水中浓缩病毒的能力。破碎的过滤材料的ζ电位表明，用阳离子聚合物处理后，带负电的环氧玻璃纤维过滤器的电正性增强。一般来说，发现用阳离子聚合物处理的环氧玻璃纤维过滤器比未处理的过滤器吸附更大比例的大肠杆菌噬菌体和肠道病毒。
