Farrah S R, Preston D R, Toranzos G A, Girard M, Erdos G A, Vasuhdivan V
Department of Microbiology and Cell Science and Engineering, University of Florida, Gainesville 32611-0100.
Appl Environ Microbiol. 1991 Sep;57(9):2502-6. doi: 10.1128/aem.57.9.2502-2506.1991.
Diatomaceous earth was modified by in situ precipitation of metallic hydroxides. Modification decreased the negative charge on the diatomaceous earth and increased its ability to adsorb viruses in water. Electrostatic interactions were more important than hydrophobic interactions in virus adsorption to modified diatomaceous earth. Filters containing diatomaceous earth modified by in situ precipitation of a combination of ferric chloride and aluminum chloride adsorbed greater than 80% of enteroviruses (poliovirus 1, echovirus 5, and coxsackievirus B5) and coliphage MS2 present in tap water at ambient pH (7.8 to 8.3), even after filtration of 100 liters of tap water. Viruses adsorbed to the filters could be recovered by mixing the modified diatomaceous earth with 3% beef extract plus 1 M NaCl (pH 9).
通过金属氢氧化物的原位沉淀对硅藻土进行改性。改性降低了硅藻土上的负电荷，并提高了其对水中病毒的吸附能力。在病毒吸附到改性硅藻土的过程中，静电相互作用比疏水相互作用更为重要。含有通过氯化铁和氯化铝组合原位沉淀改性的硅藻土的过滤器，在环境pH值（7.8至8.3）下，能够吸附自来水中超过80%的肠道病毒（脊髓灰质炎病毒1型、埃可病毒5型和柯萨奇病毒B5型）以及噬菌体MS2，即使在过滤100升自来水之后也是如此。吸附在过滤器上的病毒可以通过将改性硅藻土与3%牛肉提取物加1 M NaCl（pH 9）混合来回收。
