Goyal S M, Zerda K S, Gerba C P
Appl Environ Microbiol. 1980 Jan;39(1):85-91. doi: 10.1128/aem.39.1.85-91.1980.
Membrane filter adsorption-elution technology has been extensively used for the concentration and detection of animal viruses from large volumes of water. This study describes the development of positively charged microporous filters (Zeta Plus) for the concentration of coliphages from large volumes of water and wastewater. Four different coliphages were studied: MS-2, phi X174, T2, and T4. Positively charged microporous filters were found to efficiently adsorb these coliphages from tap water, sewage, and lake water at neutral pH. Adsorbed viruses were eluted with a 1:1 mixture of 8% beef extract and 1 M sodium chloride at pH 9. Using this method, coliphages could be concentrated from 17-liter volumes of tap water with recoveries ranging from 34 to 100%. Coliphages occurring naturally in raw and secondarily treated sewage were recovered with average efficiencies of 56.5 and 55.0%, respectively. This method should be useful in isolation of rare phages, the ecology of phages in natural waters, and the evaluation of water quality.
膜过滤吸附 - 洗脱技术已被广泛用于从大量水中浓缩和检测动物病毒。本研究描述了用于从大量水和废水中浓缩大肠杆菌噬菌体的带正电荷微孔过滤器（Zeta Plus）的开发。研究了四种不同的大肠杆菌噬菌体：MS - 2、phi X174、T2和T4。发现带正电荷的微孔过滤器在中性pH值下能有效地从自来水、污水和湖水中吸附这些大肠杆菌噬菌体。吸附的病毒在pH值为9时用8%牛肉提取物和1 M氯化钠的1:1混合物洗脱。使用这种方法，可以从17升自来水中浓缩大肠杆菌噬菌体，回收率在34%至100%之间。在原污水和二级处理污水中天然存在的大肠杆菌噬菌体的回收率分别平均为56.5%和55.0%。这种方法在稀有噬菌体的分离、天然水体中噬菌体的生态学以及水质评估方面应该是有用的。
