根据平衡热力学力比估算的线粒体复合物I的质子/电子化学计量比。
Proton/electron stoichiometry of mitochondrial complex I estimated from the equilibrium thermodynamic force ratio.
作者信息
Brown G C, Brand M D
机构信息
Department of Biochemistry, University of Cambridge, U.K.
出版信息
Biochem J. 1988 Jun 1;252(2):473-9. doi: 10.1042/bj2520473.
Abstract
The thermodynamic forces on electrons (delta Eh) and protons (delta p) across mitochondrial complexes I, III and IV were measured in isolated mitochondria respiring on succinate. The force ratio (delta Eh/delta p) across complex I close to equilibrium was found to be about 2. The equilibrium force ratio across complex I was measured during sulphite oxidation and was again close to 2. These results indicate that the proton/electron stoichiometry of complex I is 2, in conditions of high protonmotive force.
摘要
在以琥珀酸为呼吸底物的离体线粒体中，测量了跨线粒体复合物I、III和IV的电子（ΔEh）和质子（Δp）的热力学力。发现在接近平衡时，跨复合物I的力比（ΔEh/Δp）约为2。在亚硫酸盐氧化过程中测量了跨复合物I的平衡力比，其同样接近2。这些结果表明，在高质子动力势条件下，复合物I的质子/电子化学计量比为2。
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