灵芝孢子中高度支化水溶性 β-葡聚糖的结构表征及免疫增强活性。
Structural characterization and immuno-enhancing activity of a highly branched water-soluble β-glucan from the spores of Ganoderma lucidum.
机构信息
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Shanghai 201403, China; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Shanghai 201403, China.
出版信息
Carbohydr Polym. 2017 Jul 1;167:337-344. doi: 10.1016/j.carbpol.2017.03.016. Epub 2017 Mar 9.
A water soluble β-glucan (GLSWA-I) with a weight average molecular weight ∼1.57×10g/mol, isolated from the aqueous extract of the broken cellular wall Ganoderma lucidum spores, was purified by anion-exchange and gel-permeation chromatography. The immunological activity test in vivo showed that GLSWA-I could significantly promote dinitrochlorobenzene (DNCB) induced delayed-type ear swelling in mice. Based on the monosaccharides composition analysis, methylation analysis, IR, 1D and 2D NMR spectroscopy, the repeating unit of polysaccharide GLSWA-I was elucidated as follows.
一种水溶性β-葡聚糖（GLSWA-I），其重均分子量约为 1.57×10g/mol，从灵芝孢子破壁细胞提取液中分离得到，通过阴离子交换和凝胶渗透色谱进行纯化。体内免疫活性试验表明，GLSWA-I 可显著促进二硝基氯苯（DNCB）诱导的小鼠迟发型耳部肿胀。根据单糖组成分析、甲基化分析、IR、1D 和 2D NMR 光谱，多糖 GLSWA-I 的重复单元如下所示。
Zotero 插件