Abdessamad Itto Rahou, Aboubaker El-Hessni, Youssef Bahbiti, Leila Bikjdaouene, Fatima Azeroil, Youssef El Mekhlouf, Laila Ibouzine-Dine, Abdelhalem Mesfioui
Laboratory of Biology and Health, Neurosciences, Neuroimmunology and Behavior Unit, Faculty of Science, Ibn Tofail University, Kenitra, Morocco.
Higher Institute of Nursing and Health Technology of Tangier (ISPITS), Tangier, Morocco.
Neurochem Res. 2025 Sep 3;50(5):279. doi: 10.1007/s11064-025-04536-2.
Ganoderma lucidum extract (GLE) has neuro-therapeutic and anticonvulsant effects. This study aimed to evaluate the potential neuroprotective and antioxidant effects of ethanolic extract on pentylenetetrazol-induced brain damage in male Wistar rats and to analyze behavioral manifestations in vivo. Seizure latency, duration, and severity were measured in PTZ-treated rats according to the Racine scale; thus, oxidative stress markers, malondialdehyde (MDA), nitric oxide (NO), and catalase catalytic activity were measured by the 2-thiobarbituric acid, Griess reagent, and hydrogen peroxide methods, respectively. PTZ-injured rats showed both shorter seizure latency and longer seizure duration and intense seizure severity. The effects were enhanced by GLE in a dose-dependent manner. In addition, GLE decreased NO levels, increased catalase levels and decreased MDA levels in the hippocampus and the prefrontal cortex in rats treated with PTZ (75 mg/kg; i.p.). GLE conferred anticonvulsant and neuroprotective effects against PTZ-induced brain damage by repressing molecular processes involved in the production of oxidative stress markers.
灵芝提取物（GLE）具有神经治疗和抗惊厥作用。本研究旨在评估乙醇提取物对雄性Wistar大鼠戊四氮诱导的脑损伤的潜在神经保护和抗氧化作用，并分析体内行为表现。根据拉辛量表测量PTZ处理大鼠的癫痫发作潜伏期、持续时间和严重程度；因此，分别通过2-硫代巴比妥酸、格里斯试剂和过氧化氢法测量氧化应激标志物丙二醛（MDA）、一氧化氮（NO）和过氧化氢酶催化活性。PTZ损伤的大鼠癫痫发作潜伏期缩短，发作持续时间延长，发作严重程度增强。GLE以剂量依赖的方式增强了这些作用。此外，GLE降低了PTZ（75mg/kg；腹腔注射）处理大鼠海马和前额叶皮质中的NO水平，提高了过氧化氢酶水平，降低了MDA水平。GLE通过抑制氧化应激标志物产生所涉及的分子过程，对PTZ诱导的脑损伤具有抗惊厥和神经保护作用。
