Chen Huiping, Zhao Mouming, Lin Lianzhu, Wang Jufang, Sun-Waterhouse Dongxiao, Dong Yi, Zhuang Mingzhu, Su Guowan
College of Light Industry and Food Sciences, South China University of Technology, China.
College of Light Industry and Food Sciences, South China University of Technology, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510650, China.
Food Res Int. 2015 Dec;78:216-223. doi: 10.1016/j.foodres.2015.10.008. Epub 2015 Oct 9.
Defatted walnut meal (DWM), a main byproduct of walnut oil production, is rich in proteins with a high essential amino acid content. Our research was focused on the functional activities of defatted walnut meal hydrolysate (DWMH). DWMH exhibited relatively strong hydroxyl scavenging and oxygen radical absorbance capacities, protective effect on HO-injured PC12 cells compared with GSH and cerebrolysin. Besides, DWMH could combat d-galactose induced learning and memory impairments in the Morris water maze test and in the Dark/light avoidance test of mice. Seventy-seven peptides were identified by UPLC-ESI-MS/MS in the most potent antioxidative fraction of DWMH. WSREEQEREE and ADIYTEEAGR were the peptides that most likely accounted for the suppression of HO-induced apoptosis in PC12 cells. The mixture of the commercially available amino acids did not offer any protection on HO-injured PC12 cells, suggesting that amino acid sequence rather than amino acid composition was the dominant influencing factor. DWMH could be recommended as natural antioxidants for the development of functional foods targeting memory impairments.
脱脂核桃粕（DWM）是核桃油生产的主要副产品，富含蛋白质，必需氨基酸含量高。我们的研究集中在脱脂核桃粕水解物（DWMH）的功能活性上。与谷胱甘肽和脑蛋白水解物相比，DWMH表现出较强的羟自由基清除能力和氧自由基吸收能力，对过氧化氢损伤的PC12细胞具有保护作用。此外，在小鼠的莫里斯水迷宫试验和明暗回避试验中，DWMH可以对抗D-半乳糖诱导的学习和记忆障碍。通过UPLC-ESI-MS/MS在DWMH最有效的抗氧化部分鉴定出77种肽。WSREEQEREE和ADIYTEEAGR是最有可能抑制过氧化氢诱导的PC12细胞凋亡的肽。市售氨基酸混合物对过氧化氢损伤的PC12细胞没有任何保护作用，这表明氨基酸序列而非氨基酸组成是主要影响因素。DWMH可作为天然抗氧化剂推荐用于开发针对记忆障碍的功能性食品。
