Li Wenzhi, Zhao Tiantian, Zhang Jianan, Xu Jucai, Sun-Waterhouse Dongxiao, Zhao Mouming, Su Guowan
School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640 China.
Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou, 510650 China.
J Food Sci Technol. 2017 Sep;54(10):3102-3110. doi: 10.1007/s13197-017-2746-x. Epub 2017 Jul 5.
A walnut protein hydrolysate (WPH) was prepared by using a mixture of pancreatin and viscozyme L from industrially available defatted walnut meal. The antioxidant effects of WPH were confirmed and quantified by reducing power, oxygen radical absorbance capacity, hydroxyl radical radical-scavenging activity and ABTS· radical-scavenging activity assays. The protective effects of WPH on scopolamine-induced learning and memory deficits in mice were also evaluated based on in vivo behavioral tests. Results showed that WPH administration would lead to significantly decreased latencies while increased crossing times and target times in the spatial probe test, and increased escape latency and decreased error times in the step-down avoidance test for the scopolamine-induced dementia mice. Biochemical results indicated that the ameliorative effects of WPH on scopolamine-induced dementia mice could be attributed to the significantly increased amount of acetylcholine receptors. Therefore, WPH may be a potential therapeutic agent against Alzheimer's disease.
采用胰蛋白酶和里氏木霉的混合物，从市售脱脂核桃粕中制备了核桃蛋白水解物（WPH）。通过还原能力、氧自由基吸收能力、羟自由基清除活性和ABTS·自由基清除活性测定，对WPH的抗氧化作用进行了确认和定量。还基于体内行为测试评估了WPH对东莨菪碱诱导的小鼠学习和记忆缺陷的保护作用。结果表明，对于东莨菪碱诱导的痴呆小鼠，给予WPH会导致空间探索试验中的潜伏期显著缩短，同时穿越次数和目标象限停留时间增加，在跳台回避试验中逃避潜伏期增加且错误次数减少。生化结果表明，WPH对东莨菪碱诱导的痴呆小鼠的改善作用可能归因于乙酰胆碱受体数量的显著增加。因此，WPH可能是一种潜在的抗阿尔茨海默病治疗剂。
