Halldorsdottir Valgerdur G, Dave Jaydev K, Marshall Andrew, Forsberg Anya I, Fox Traci B, Eisenbrey John R, Machado Priscilla, Liu Ji-Bin, Merton Daniel A, Forsberg Flemming
Department of Radiology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA; School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, Pennsylvania, USA.
Department of Radiology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
Ultrasound Med Biol. 2017 Jul;43(7):1401-1410. doi: 10.1016/j.ultrasmedbio.2017.02.011. Epub 2017 Apr 19.
Interstitial fluid pressure (IFP) in rats with breast cancer xenografts was non-invasively estimated using subharmonic-aided pressure estimation (SHAPE) versus an invasive pressure monitor. Moreover, monitoring of IFP changes after chemotherapy was assessed. Eighty-nine rats (calibration n = 25, treatment n = 64) were injected with 5 × 10 breast cancer cells (MDA-MB-231). Radiofrequency signals were acquired (39 rats successfully imaged) with a Sonix RP scanner (BK Ultrasound, Richmond, BC, Canada) using a linear array (L9-4, transmit/receive: 8/4 MHz) after administration of Definity (Lantheus Medical Imaging, North Billerica, MA, USA; 180 μL/kg) and compared with readings from an invasive pressure monitor (Stryker, Berkshire, UK). An inverse linear relationship was established between tumor IFP and SHAPE (y = -1.06x + 28.27, r = -0.69, p = 0.01) in the calibration group. Use of this relationship in the treatment group resulted in r = 0.74 (p < 0.05) between measured (pressure monitor) and SHAPE-estimated IFP (average error: 6.24 mmHg). No significant before/after differences were observed with respect to paclitaxel treatment (5 mg/kg, Mayne Pharma, Paramus, NJ, USA) with either method (p ≥ 0.15).
使用次谐波辅助压力估计（SHAPE）与侵入性压力监测器对乳腺癌异种移植大鼠的组织间液压力（IFP）进行了非侵入性估计。此外，还评估了化疗后IFP变化的监测情况。89只大鼠（校准组n = 25，治疗组n = 64）被注射了5×10个乳腺癌细胞（MDA-MB-231）。在给予Definity（美国马萨诸塞州北比勒里卡市Lantheus Medical Imaging公司；180μL/kg）后，使用线性阵列（L9-4，发射/接收：8/4 MHz）的Sonix RP扫描仪（加拿大不列颠哥伦比亚省里士满市BK超声公司）采集射频信号（39只大鼠成功成像），并与侵入性压力监测器（英国伯克郡Stryker公司）的读数进行比较。在校准组中，肿瘤IFP与SHAPE之间建立了反线性关系（y = -1.06x + 28.27，r = -0.69，p = 0.01）。在治疗组中使用这种关系，测量的（压力监测器）与SHAPE估计的IFP之间的r = 0.74（p < 0.05）（平均误差：6.24 mmHg）。两种方法在紫杉醇治疗（5 mg/kg，美国新泽西州帕拉默斯市Mayne Pharma公司）前后均未观察到显著差异（p≥0.15）。
