Fardsanei Fatemeh, Soltan Dallal Mohammad Mehdi, Douraghi Masoumeh, Zahraei Salehi Taghi, Mahmoodi Mahmood, Memariani Hamed, Nikkhahi Farhad
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran.
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2017 Jun;107:451-456. doi: 10.1016/j.micpath.2017.04.026. Epub 2017 Apr 20.
Salmonella enterica subspecies enterica serotype Enteritidis (S. Enteritidis) is one of the leading causes of food-borne gastroenteritis associated with the consumption of contaminated food products of animal origin. Little is known about the genetic diversity and virulence content of S. Enteritidis isolated from poultry meats and eggs in Iran. A total of 34 S. Enteritidis strains were collected from different food sources of animal origin in Tehran from May 2015 to July 2016. All of the S. Enteritidis strains were serotyped, antimicrobial susceptibility tested, and characterized for virulence genes. Pulsed-field gel electrophoresis (PFGE) was also applied for comparison of genetic relatedness. All of the strains harbored invA, hilA, ssrA, sefA, spvC, and sipA genes. A high prevalence of resistance against certain antibiotics such as cefuroxime (79.4%), nalidixic acid (47%), and ciprofloxacin (44.2%) was also observed. Regarding PFGE, S. Enteritidis strains from different sources showed considerable overlap, suggesting the lack of diversity among these isolates. Moreover, no correlation between virulence profiles or antibiotypes and PFGE clusters was observed. In conclusion, our study provided valuable information on virulence gene content, antibiotic resistance, and genetic diversity of S. Enteritidis isolated from food sources.
肠炎沙门氏菌肠炎亚种肠炎血清型（肠炎沙门氏菌）是与食用受污染的动物源性食品相关的食源性肠胃炎的主要病因之一。关于从伊朗禽肉和蛋类中分离出的肠炎沙门氏菌的遗传多样性和毒力成分，人们了解甚少。2015年5月至2016年7月期间，从德黑兰不同动物源性食物来源共收集了34株肠炎沙门氏菌菌株。对所有肠炎沙门氏菌菌株进行了血清分型、抗菌药敏试验，并对毒力基因进行了鉴定。还应用脉冲场凝胶电泳（PFGE）比较遗传相关性。所有菌株均携带invA、hilA、ssrA、sefA、spvC和sipA基因。还观察到对某些抗生素如头孢呋辛（79.4%）、萘啶酸（47%）和环丙沙星（44.2%）的高耐药率。关于PFGE，来自不同来源的肠炎沙门氏菌菌株显示出相当大的重叠，表明这些分离株之间缺乏多样性。此外，未观察到毒力谱或抗菌谱与PFGE聚类之间的相关性。总之，我们的研究提供了关于从食物来源分离出的肠炎沙门氏菌的毒力基因含量、抗生素耐药性和遗传多样性的有价值信息。
