α-人心房利钠多肽抑制人肾上腺细胞分泌19-羟雄烯二酮。

Alpha-human atrial natriuretic polypeptide inhibits 19-hydroxy-androstenedione secretion by human adrenal cells.

作者信息

Higuchi K, Nawata H, Kato K, Ibayashi H

机构信息

Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

出版信息

Horm Metab Res. 1989 Feb;21(2):92-5. doi: 10.1055/s-2007-1009159.

DOI:10.1055/s-2007-1009159

PMID:2542147

Abstract

We investigated the effect of ACTH, angiotensin II (AII), and alpha-human atrial natriuretic polypeptide (alpha-hANP) which plays an important role of water-electrolytes balance, on 19-hydroxyandrostenedione (19-hydroxyandrost-4-ene-3,17-dione, 19-OH-A-dione) secretion by cultured human adrenal cells. 19-OH-A-dione in culture media was measured using a specific RIA. Basal 19-OH-A-dione secretion by adrenal cells was 0.69 +/- 0.08 ng/3h/10(6) cells and significantly rose to 1.17 +/- 0.14 ng/3h/10(6) cells in the presence of ACTH, but not in the presence of A II. These results demonstrate that 196-OH-A-dione is directly secreted from adrenal cells. alpha-hANP significantly inhibited both basal and ACTH-stimulated 19-OH-A-dione secretions, as well as aldosterone. These results demonstrate that alpha-hANP inhibits aldosterone activity by means of the inhibition of both aldosterone and 19-OH-A-dione (an aldosterone amplifier) secretion by adrenal cells.

摘要

我们研究了促肾上腺皮质激素（ACTH）、血管紧张素II（AII）以及在水电解质平衡中起重要作用的α-人心房利钠肽（α-hANP）对培养的人肾上腺细胞分泌19-羟雄烯二酮（19-羟基-4-烯-3,17-二酮，19-OH-A-二酮）的影响。使用特异性放射免疫分析法测定培养基中的19-OH-A-二酮。肾上腺细胞基础状态下19-OH-A-二酮的分泌量为0.69±0.08 ng/3小时/10⁶个细胞，在存在ACTH时显著升高至1.17±0.14 ng/3小时/10⁶个细胞，但在存在AII时无显著变化。这些结果表明19-OH-A-二酮是由肾上腺细胞直接分泌的。α-hANP显著抑制基础状态下以及ACTH刺激下的19-OH-A-二酮分泌，同时也抑制醛固酮的分泌。这些结果表明，α-hANP通过抑制肾上腺细胞分泌醛固酮和19-OH-A-二酮（一种醛固酮增强剂）来抑制醛固酮活性。