人细小病毒B19：酶联免疫吸附测定和免疫印迹分析

Human parvovirus B19: ELISA and immunoblot assays.

作者信息

Schwarz T F, Roggendorf M, Deinhardt F

机构信息

Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, F.R.G.

出版信息

J Virol Methods. 1988 Jun;20(2):155-68. doi: 10.1016/0166-0934(88)90149-8.

DOI:10.1016/0166-0934(88)90149-8

PMID:2843558

Abstract

An ELISA for the detection of specific IgM and IgG against human parvovirus B19 (anti-B19 IgM and IgG) and B19 antigen is described. With ELISA anti-B19 IgM could be detected for up to 20 weeks after viraemia. Four to five months after B19 infection anti-B19 IgG titres range between 10(-6) and 10(-7). Nonspecific reactions with rheumatoid factor or IgM against rubella were not found. The ELISA for B19 antigen was shown to be as sensitive as DNA hybridisation. With immunoblotting two viral proteins of 83 kd (VP1) and 58 kd (VP2) were demonstrated. After acute infection antibodies to VP2 appear before antibodies to VP1. Immunoblotting might be used in pregnancy to determine the time of maternal infection. In a survey of a B19 outbreak in a school for medical technology, 6 (28.6%) of 21 non-immune females seroconverted.

摘要

本文描述了一种用于检测抗人细小病毒B19特异性IgM和IgG（抗B19 IgM和IgG）以及B19抗原的酶联免疫吸附测定（ELISA）。通过ELISA，在病毒血症后长达20周都可检测到抗B19 IgM。B19感染后4至5个月，抗B19 IgG滴度在10^(-6)至10^(-7)之间。未发现与类风湿因子或抗风疹IgM的非特异性反应。检测B19抗原的ELISA与DNA杂交一样灵敏。通过免疫印迹法证实了两种病毒蛋白，分别为83kd（VP1）和58kd（VP2）。急性感染后，抗VP2抗体先于抗VP1抗体出现。免疫印迹法可用于孕期确定母体感染时间。在对一所医学技术学校中B19爆发情况的调查中，21名无免疫力的女性中有6名（28.6%）发生了血清转化。