Schwarz T F, Jäger G, Gilch S, Pauli C
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, Munich, Germany.
Epidemiol Infect. 1995 Jun;114(3):501-10. doi: 10.1017/s0950268800052213.
Of eight acute infections in German tourists caused by sandfly fever virus, serotype Toscana (TOS), and diagnosed clinically and serologically, seven were acquired during visits to Tuscany, Italy, and one to Coimbra, Portugal. An indirect immunofluorescence assay (IFA) using infected cells, and a newly developed enzyme-immunoassay (EIA) using crude virus antigen prepared from infected Vero-E6 cells was used to detect anti-TOS IgM and IgG. In a seroepidemiological survey of 859 health care workers and medical students, anti-TOS IgG was detected in 1.0% by IFA, and in 0.7% by EIA. In 2034 German patients hospitalized for various diseases, 1.6% were positive for anti-TOS IgG by IFA, and 0.8% by EIA. Anti-TOS IgG was detected in 43 samples of commercial immunoglobulins at titres of 10-1000 by EIA. Although the seroprevalence of antibodies to TOS is low in Germany, TOS infection should be considered in patients returning from endemic areas who complain of fever, and headaches, and have symptoms of meningitis.
在8名由托斯卡纳血清型（TOS）白蛉热病毒引起急性感染的德国游客中，经临床和血清学诊断，7例是在访问意大利托斯卡纳期间感染的，1例是在访问葡萄牙科英布拉期间感染的。使用感染细胞的间接免疫荧光法（IFA）以及使用从感染的Vero-E6细胞制备的粗病毒抗原新开发的酶免疫法（EIA）来检测抗TOS IgM和IgG。在一项对859名医护人员和医学生的血清流行病学调查中，通过IFA检测到抗TOS IgG的比例为1.0%，通过EIA检测到的比例为0.7%。在2034名因各种疾病住院的德国患者中，通过IFA检测到抗TOS IgG阳性的比例为1.6%，通过EIA检测到的比例为0.8%。通过EIA在43份商业免疫球蛋白样本中检测到抗TOS IgG，滴度为10 - 1000。尽管德国人群中抗TOS抗体的血清阳性率较低，但对于从流行地区返回且主诉发热、头痛并有脑膜炎症状的患者，应考虑TOS感染。
