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一种用于……的简单且高效的介导转化方案。 (注:原文中“-mediated transformation protocol for.”部分内容不完整,翻译可能不太准确,完整准确的翻译需补充完整原文信息。)

A simple and highly efficient -mediated transformation protocol for .

作者信息

Martins Polyana Kelly, Ribeiro Ana Paula, Cunha Bárbara Andrade Dias Brito da, Kobayashi Adilson Kenji, Molinari Hugo Bruno Correa

机构信息

Genetics and Biotechnology Laboratory, Embrapa Agroenergy, Brasília, DF, Brazil.

出版信息

Biotechnol Rep (Amst). 2015 Feb 18;6:41-44. doi: 10.1016/j.btre.2015.02.002. eCollection 2015 Jun.

Abstract

The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, is being tested as a model plant for sugarcane. has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for -mediated genetic transformation of . The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane.

摘要

甘蔗在巴西的生产和利用对于生物能源生产非常重要,并且被认为是世界上效率最高的之一。在我们实验室,[具体植物名称未给出]正被作为甘蔗的模式植物进行测试。[具体植物名称未给出]具有一些生物学特性(生命周期短、基因组小、二倍体、植株矮小且生长要求简单),使其适合用作模式系统。我们报道了一种用于[具体植物名称未给出]的农杆菌介导遗传转化的高效方案。组织培养中几个步骤的优化使得植物能够快速再生,并将转化效率提高到了29%。该方案可能成为甘蔗功能基因组学的有力工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f1c/5374281/77bbfa92e5d9/gr1.jpg

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