Martins Polyana Kelly, Ribeiro Ana Paula, Cunha Bárbara Andrade Dias Brito da, Kobayashi Adilson Kenji, Molinari Hugo Bruno Correa
Genetics and Biotechnology Laboratory, Embrapa Agroenergy, Brasília, DF, Brazil.
Biotechnol Rep (Amst). 2015 Feb 18;6:41-44. doi: 10.1016/j.btre.2015.02.002. eCollection 2015 Jun.
The production and use of sugarcane in Brazil is very important for bioenergy production and is recognized as one of the most efficient in the world. In our laboratory, is being tested as a model plant for sugarcane. has biological attributes (rapid life cycle, small genome, diploid, short stature and simple growth requirements) that make it suitable for use as a model system. We report a highly efficient protocol for -mediated genetic transformation of . The optimization of several steps in tissue culture allowed the rapid regeneration of plants and increased the rate of transformation up to 29%. This protocol could become a powerful tool for functional genomics in sugarcane.
甘蔗在巴西的生产和利用对于生物能源生产非常重要，并且被认为是世界上效率最高的之一。在我们实验室，[具体植物名称未给出]正被作为甘蔗的模式植物进行测试。[具体植物名称未给出]具有一些生物学特性（生命周期短、基因组小、二倍体、植株矮小且生长要求简单），使其适合用作模式系统。我们报道了一种用于[具体植物名称未给出]的农杆菌介导遗传转化的高效方案。组织培养中几个步骤的优化使得植物能够快速再生，并将转化效率提高到了29%。该方案可能成为甘蔗功能基因组学的有力工具。
