Siirt University, Faculty of Art and Science, Department of Biology, Siirt, 56100, Turkey.
The University of Veterinary and Animal Sciences, Institute of Biochemistry and Biotechnology, Lahore, 45650, Pakistan.
Anal Biochem. 2022 Mar 15;641:114566. doi: 10.1016/j.ab.2022.114566. Epub 2022 Jan 25.
Fungi can be used as a potent chemotherapeutic agent to treat various cancers. In current study acetone and methanol extracts of Terfezia claveryi, Terfezia boudieri, Terfezia olbiensis, Picoa lefebvrei, Picoa juniperi were used to assess total phenolic contents, antioxidant activity, ion-chelating impact, antimicrobial activity, the cytotoxic and protective effects. Both methanol and acetone extracts of T. boudieri had the highest FRAP and DPPH scavenging abilities. Dose-dependent increased ion-chelating impact of all tested truffles species was found. Extracts of T. boudieri, T. claveryi, and T. albiensis exhibited higher antimicrobial activities. T. claveryi and T. boudieri showed the highest protective effects against HO-induced genotoxicity (P < 0.05), in S. cerevisiae BY4741. The least protective effect was showed by the acetone extracts of T. olbiensis (144 ± 8); methanol extracts of P. lefebvrei (140 ± 8) and P. juniperi (140 ± 10). MCF 7 cells showed more sensitivity against to methanol extracts of T. boudieri at 10-100 μg/mL concentrations. HepG2 cells showed more sensitivity against the methanolic extracts of T. boudieri at both doses. Overall, P. lefebvrei and P. juniperi extracts had the least cytotoxic effects. The species of Terfezia exhibit significant protective effects against DNA damage and also have the potential of cytotoxicity effects.
真菌可用作治疗各种癌症的有效化疗药物。在当前的研究中，使用了 Terfezia claveryi、Terfezia boudieri、Terfezia olbiensis、Picoa lefebvrei、Picoa juniperi 的丙酮和甲醇提取物来评估总酚含量、抗氧化活性、离子螯合作用、抗菌活性、细胞毒性和保护作用。T. boudieri 的甲醇和丙酮提取物均具有最高的 FRAP 和 DPPH 清除能力。发现所有测试的块菌物种的离子螯合作用呈剂量依赖性增加。T. boudieri、T. claveryi 和 T. albiensis 的提取物表现出更高的抗菌活性。T. claveryi 和 T. boudieri 对 HO 诱导的遗传毒性表现出最高的保护作用（P<0.05），在 S. cerevisiae BY4741 中。T. olbiensis 的丙酮提取物（144±8）；P. lefebvrei（140±8）和 P. juniperi（140±10）的甲醇提取物表现出最低的保护作用。MCF 7 细胞对 10-100μg/mL 浓度的 T. boudieri 甲醇提取物更敏感。HepG2 细胞对 T. boudieri 的甲醇提取物在两种剂量下均表现出更高的敏感性。总体而言，P. lefebvrei 和 P. juniperi 提取物的细胞毒性作用最小。Terfezia 物种对 DNA 损伤具有显著的保护作用，并且具有细胞毒性作用的潜力。
