Panganiban A T
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
J Virol. 1988 Oct;62(10):3574-80. doi: 10.1128/JVI.62.10.3574-3580.1988.
In some type C retroviruses, translation of the pol gene appears to require translational suppression of the proximal gag amber codon. To identify the region of the viral nucleic acid responsible for synthesis of the pol gene products, a 300-base-pair DNA fragment containing the stop codon from a type C murine virus (AK virus) was inserted into the Escherichia coli lacZ gene such that the translational reading frame was maintained. Introduction of the resulting fusion gene into cells resulted in the suppression of the viral stop codon. As measured by beta-galactosidase production, suppression occurred at a frequency of approximately 10%. Suppression could occur in at least several vertebrate cell types and was not augmented by virus replication or the expression of viral gene products. This indicates that gag amber codon suppression does not require augmented levels of suppressor tRNA species.
在某些C型逆转录病毒中，pol基因的翻译似乎需要对近端gag琥珀密码子进行翻译抑制。为了鉴定负责pol基因产物合成的病毒核酸区域，将一个包含来自C型鼠病毒（AK病毒）的终止密码子的300个碱基对的DNA片段插入大肠杆菌lacZ基因中，从而维持翻译阅读框。将所得融合基因导入细胞导致病毒终止密码子的抑制。通过β-半乳糖苷酶产生来测量，抑制发生的频率约为10%。抑制可发生在至少几种脊椎动物细胞类型中，并且不会因病毒复制或病毒基因产物的表达而增强。这表明gag琥珀密码子抑制不需要增加抑制性tRNA种类的水平。
