Center for Pharmaceutical Sciences, Faculty of Life Science and Technology, Kunming University of Science and Technology , Chenggong Campus, Kunming 650500, P. R. China.
National Center for Natural Products Research, School of Pharmacy, University of Mississippi , Mississippi 38677, United States.
J Agric Food Chem. 2017 May 10;65(18):3702-3710. doi: 10.1021/acs.jafc.7b01350. Epub 2017 May 1.
The essential oils (EOs) derived from aromatic plants such as Piper species are considered to play a role in alleviating neuronal ailments that are associated with inhibition of acetylcholinesterase (AChE). The chemical compositions of 23 EOs prepared from 16 Piper spp. were analyzed by both gas chromatography with a flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 76 compounds were identified in the EOs from the leaves and stems of 19 samples, while 30 compounds were detected in the EOs from the fruits of four samples. Sesquiterpenes and phenylpropanoids were found to be rich in these EOs, of which asaricin, caryophyllene, caryophyllene oxide, isospathulenol, (+)-spathulenol, and β-bisabolene are the major constituents. The EOs from the leaves and stems of Piper austrosinense, P. puberulum, P. flaviflorum, P. betle, and P. hispidimervium showed strong AChE inhibitory activity with IC values in the range of 1.51 to 13.9 mg/mL. A thin-layer chromatography (TLC) bioautography assay was employed to identify active compound(s) in the most active EO from P. hispidimervium. The active compound was isolated and identified as asaricin, which gave an IC value of 0.44 ± 0.02 mg/mL against AChE, comparable to galantamine with an IC 0.15 ± 0.01 mg/mL.
从芳香植物如胡椒属植物中提取的精油（EOs）被认为在缓解与乙酰胆碱酯酶（AChE）抑制有关的神经元疾病方面发挥作用。通过气相色谱法与火焰电离检测器（GC-FID）和气相色谱-质谱联用（GC-MS）分析了 16 种胡椒属植物的 23 种精油的化学成分。在 19 个样品的叶和茎精油中鉴定出 76 种化合物，而在 4 个样品的果实精油中检测到 30 种化合物。这些精油中含有丰富的倍半萜类和苯丙素类化合物，其中包括asaricin、丁香烯、丁香烯氧化物、异spathulenol、(+)-spathulenol 和 β- bisabolene 是主要成分。来自 Piper austrosinense、P. puberulum、P. flaviflorum、P. betle 和 P. hispidimervium 的叶和茎精油对 AChE 具有很强的抑制活性，IC 值在 1.51 至 13.9 mg/mL 范围内。采用薄层层析（TLC）生物自显影法鉴定 P. hispidimervium 中最活跃的精油中的活性化合物。活性化合物被分离并鉴定为 asaricin，对 AChE 的 IC 值为 0.44 ± 0.02 mg/mL，与 IC 0.15 ± 0.01 mg/mL 的加兰他敏相当。
