叶片和花挥发油的化学成分和乙酰胆碱酯酶抑制活性研究

Chemical Composition and Anticholinesterase Activity of the Essential Oil of Leaves and Flowers from the Ecuadorian Plant (Kunth) Epling.

机构信息

Departamento de Química, Maestría en Química Aplicada, Universidad Técnica Particular de Loja (UTPL), Calle Marcelino Champagnat s/n, Loja 1101608, Ecuador.

Institute for Regenerative Medicine and Biotherapy (IRMB), Université de Montpellier, INSERM, 34295 Montpellier, France.

出版信息

Molecules. 2021 May 27;26(11):3198. doi: 10.3390/molecules26113198.

DOI:10.3390/molecules26113198

PMID:34071744

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8199004/

Abstract

This work aimed to study the chemical composition, cholinesterase inhibitory activity, and enantiomeric analysis of the essential oil from the aerial parts (leaves and flowers) of the plant (Kunth) Epling from Ecuador. The essential oil (EO) was obtained through steam distillation. The chemical composition of the oil was evaluated by gas chromatography, coupled to mass spectrometry (GC-MS) and a flame ionization detector (GC-FID). The analyses led to the identification of 69 compounds in total, of which 40 were found in the leaves and 29 were found in the flowers of the plant. The major components found in the oil were 1,8-Cineole, β-Pinene, δ-3-Carene, α-Pinene, ()-Caryophyllene, Guaiol, and β-Phellandrene. Flower essential oil showed interesting selective inhibitory activity against both enzymes AChE (28.2 ± 1.8 2 µg/mL) and BuChE (28.8 ± 1.5 µg/mL). By contrast, the EO of the leaves showed moderate mean inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), with IC values of 38.2 ± 2.9 µg/mL and 47.4 ± 2.3 µg/mL, respectively.

摘要

这项工作旨在研究来自厄瓜多尔植物（Kunth） Epling 的地上部分（叶和花）精油的化学成分、乙酰胆碱酯酶抑制活性和对映体分析。通过水蒸气蒸馏获得精油。通过气相色谱、质谱联用（GC-MS）和火焰离子化检测器（GC-FID）评估油的化学成分。分析共鉴定出 69 种化合物，其中 40 种存在于叶中，29 种存在于花中。油中的主要成分是 1,8-桉树脑、β-蒎烯、δ-3-蒈烯、α-蒎烯、（）-石竹烯、愈创木薁和β-水芹烯。花精油对两种酶 AChE（28.2 ± 1.82 µg/mL）和 BuChE（28.8 ± 1.5 µg/mL）均显示出有趣的选择性抑制活性。相比之下，叶精油对乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）具有中等的平均抑制活性，IC 值分别为 38.2 ± 2.9 µg/mL 和 47.4 ± 2.3 µg/mL。