Wenzel R, Herrmann R
University of Heidelberg, FRG.
Nucleic Acids Res. 1988 Sep 12;16(17):8323-36. doi: 10.1093/nar/16.17.8323.
In order to study the genome organization of Mycoplasma pneumoniae a cosmid library of M. pneumoniae DNA was established using a newly designed cosmid vector (pcosRW2). From this library 32 overlapping clones were isolated covering a contiguous 720 kbp DNA segment representing about 90% of the genome assuming a genome size of about 800 kbp.
为了研究肺炎支原体的基因组结构，使用新设计的黏粒载体（pcosRW2）构建了肺炎支原体DNA的黏粒文库。从该文库中分离出32个重叠克隆，覆盖了一个连续的720kbp DNA片段，假设基因组大小约为800kbp，则该片段代表了约90%的基因组。
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