Wenzel R, Herrmann R
Department of Microbiology, University of Heidelberg, FRG.
Nucleic Acids Res. 1989 Sep 12;17(17):7029-43. doi: 10.1093/nar/17.17.7029.
The complete genome of Mycoplasma pneumoniae was cloned in an ordered library consisting of 34 overlapping or adjacent cosmids, one plasmid and two lambda phages. The genome size was determined by adding up the sizes of either the individual unique EcoRI restriction fragments of the gene bank or of the XhoI fragments of genomic M. pneumoniae DNA. The values from these calculations, 835 and 849 kbp, are in good agreement. An XhoI restriction map was constructed by identifying adjacent DNA fragments by probing with selected cosmid clones.
肺炎支原体的完整基因组被克隆到一个有序文库中，该文库由34个重叠或相邻的黏粒、一个质粒和两个λ噬菌体组成。通过将基因文库中各个独特的EcoRI限制性片段的大小或肺炎支原体基因组DNA的XhoI片段的大小相加来确定基因组大小。这些计算得出的值分别为835和849千碱基对，两者吻合良好。通过用选定的黏粒克隆进行探针杂交来鉴定相邻的DNA片段，从而构建了一个XhoI限制性图谱。
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