肽基转移酶中心与翻译系统的起源
Peptidyl Transferase Center and the Emergence of the Translation System.
作者信息
Torres de Farias Savio, Gaudêncio Rêgo Thais, José Marco V
机构信息
Laboratório de Genética Evolutiva Paulo Leminsk, Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa 58051-900, Brazil.
Departamento de Informática, Universidade Federal da Paraíba, João Pessoa 58051-900, Brazil.
出版信息
Life (Basel). 2017 Apr 25;7(2):21. doi: 10.3390/life7020021.
In this work, the three-dimensional (3D) structure of the ancestral Peptidyl Transferase Center (PTC) built by concatamers of ancestral sequences of tRNAs was reconstructed, and its possible interactions with tRNAs molecules were analyzed. The 3D structure of the ancestral PTC was also compared with the current PTC of T. thermophilus. Docking experiments between the ancestral PTC and tRNAs suggest that in the origin of the translation system, the PTC functioned as an adhesion center for tRNA molecules. The approximation of tRNAs charged with amino acids to the PTC permitted peptide synthesis without the need of a genetic code.
在这项工作中，通过tRNA祖先序列的串联体构建了祖先肽基转移酶中心（PTC）的三维（3D）结构，并分析了其与tRNA分子可能的相互作用。还将祖先PTC的3D结构与嗜热栖热菌当前的PTC进行了比较。祖先PTC与tRNA之间的对接实验表明，在翻译系统起源时，PTC作为tRNA分子的粘附中心发挥作用。携带氨基酸的tRNA与PTC的接近使得肽合成无需遗传密码。
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