Muller D, Quantin B, Gesnel M C, Millon-Collard R, Abecassis J, Breathnach R
Centre Régional de Lutte contre le Cancer Paul Strauss, Unité 184 de Biologíe Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
Biochem J. 1988 Jul 1;253(1):187-92. doi: 10.1042/bj2530187.
Stromelysin is a collagenase-related connective-tissue-degrading metalloproteinase. We have detected RNAs capable of hybridizing to a rat stromelysin cDNA in 11 of 69 human tumours tested. Molecular cloning of cDNAs to these RNAs has identified them as a mixture of stromelysin RNA and a transcript of a hitherto-undescribed related human gene, the stromelysin-2 gene. We have also isolated cDNAs corresponding to a more distantly related new human gene, the pump-1 gene. A comparison of the cDNA-derived amino acid sequences of stromelysin-2 and pump-1 with the known sequences of stromelysin and collagenase reveals significant similarities, with conservation of sequence motifs believed to have functional importance in metalloproteinase action. We conclude that the collagenase gene family in humans consists of at least four members, and speculate that expression of these genes plays a role in cancer.
基质溶解素是一种与胶原酶相关的可降解结缔组织的金属蛋白酶。在检测的69个人类肿瘤中，我们在11个肿瘤中检测到了能够与大鼠基质溶解素cDNA杂交的RNA。对这些RNA的cDNA进行分子克隆后发现，它们是基质溶解素RNA与一个此前未描述的相关人类基因（基质溶解素-2基因）转录本的混合物。我们还分离出了与一个亲缘关系更远的新人类基因（泵-1基因）相对应的cDNA。将基质溶解素-2和泵-1的cDNA推导的氨基酸序列与基质溶解素和胶原酶的已知序列进行比较，发现了显著的相似性，序列基序得以保留，据信这些基序在金属蛋白酶的作用中具有功能重要性。我们得出结论，人类的胶原酶基因家族至少由四个成员组成，并推测这些基因的表达在癌症中起作用。
